Calibration curves for biological dosimetry by fluorescence in situ hybridisation.
Calibration curves for biological dosimetry by fluorescence in situ hybridisation.
复制标题
荧光原位杂交生物剂量测定的校准曲线。
DOI：
10.1093/oxfordjournals.rpd.a006508
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发表时间：
2001
影响因子：
1
通讯作者：
C. Tanzarella
中科院分区：
文献类型：
--
作者：
L. Stronati;M. Durante;G. Gensabella;G. Gialanella;G. F. Gross;M. Pugliese;P. Scampoli;A. Sgura;A. Testa;C. Tanzarella
测定60Coγ射线急性照射后外周血淋巴细胞染色体畸变率的剂量-效应曲线。血液来自四个不同的健康献血者，染色体要么在Colcemid积累后的中期观察到，要么被Calyculin A过早浓缩。对1、2、4号染色体进行染色，在照射剂量范围为0.1~3.0Gy时，染色和未染色染色体之间出现简单的互换。化学诱导的染色体提前凝聚法结合染色体涂染(仅限4号染色体)用于确定高剂量照射(高达20GyX射线)的校准曲线。本文所描述的校准曲线将用于我们实验室的荧光原位杂交生物剂量测定。
Dose-response curves were measured for the induction of chromosomal aberrations in peripheral blood lymphocytes after acute exposure in vitro to 60Co gamma rays. Blood was obtained from four different healthy donors, and chromosomes were either observed at metaphase, following colcemid accumulation, or prematurely condensed by calyculin A. Cells were analysed in three different Italian laboratories. Chromosomes 1, 2, and 4 were painted, and simple-type interchanges between painted and non-painted chromosomes were scored in cells exposed in the dose range 0.1-3.0 Gy. The chemical-induced premature chromosome condensation method was also used combined with chromosome painting (chromosome 4 only) to determine calibration curves for high dose exposures (up to 20 Gy X rays). Calibration curves described in this paper will be used in our laboratories for biological dosimetry by fluorescence in situ hybridisation.