Transient isotachophoretic-electrophoretic separations of lanthanides with indirect laser-induced fluorescence detection.
Transient isotachophoretic-electrophoretic separations of lanthanides with indirect laser-induced fluorescence detection.
复制标题
通过间接激光诱导荧光检测对镧系元素进行瞬时等速电泳分离。
DOI：
10.1021/ac971043
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发表时间：
1998
影响因子：
7.4
通讯作者：
B. Andresen
中科院分区：
文献类型：
--
作者：
Matthew Church;J. D. Spear;R. Russo;G. Klunder;P. Grant;B. Andresen
利用间接激光诱导荧光技术对毛细管电泳法分离的几种稀土元素进行了检测。背景电解液的荧光成分为硫酸奎宁，加入α-羟基异丁酸作为络合剂，使具有相似迁移率的分析物离子能够分离。用Ar离子激光器的紫外线(333-364 nm)作为激发源，用二极管阵列探测器监测442 nm处的荧光发射。电动进样和瞬时等速电泳法用于将分析物离子堆积到更集中的区域。在线预富集系数为∼700时，La(3+)、Ce(3+)、Pr(3+)、Nd3+、Sm(3+)和Eu(3+)的检出限在低ppb范围内(6-11 nm)。
Indirect laser-induced fluorescence was used for the detection of several lanthanide species separated by capillary electrophoresis. Quinine sulfate was the fluorescent component of the background electrolyte, and α-hydroxyisobutyric acid was added as a complexing agent to enable the separation of analyte ions that have similar mobilities. The UV lines (333-364 nm) of an argon ion laser were used as the excitation source with a diode array detector for monitoring the fluorescent emission at 442 nm. Electrokinetic injections and transient isotachophoresis were implemented to stack the analyte ions into more concentrated zones. On-line preconcentration factors were determined to be ∼700 and resulted in limits of detection for La(3+), Ce(3+), Pr(3+), Nd(3+), Sm(3+), and Eu(3+) in the low-ppb range (6-11 nM).