Studies on oxidative damage induced by cyanobacteria extract in primary cultured rat hepatocytes
Studies on oxidative damage induced by cyanobacteria extract in primary cultured rat hepatocytes
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DOI：
10.1006/enrs.1998.3843
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发表时间：
1998-07-01
影响因子：
8.3
通讯作者：
Ong, CN
中科院分区：
文献类型：
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作者：
Ding, WX;Shen, HM;Ong, CN
蓝藻（蓝绿藻）对水的污染是世界各地的一个严重健康问题，主要是由于微囊藻毒素（一组强效肝毒素）的毒性作用。然而，微囊藻毒素的细胞毒性机制尚未完全阐明。在本研究中，对原代培养的大鼠肝细胞评估了冻干淡水蓝细菌提取物引起的氧化损伤。在用蓝藻提取物处理的肝细胞中观察到乳酸脱氢酶（LDH）渗漏的时间和剂量依赖性增加。脂质过氧化，氧化损伤的主要表现，也进行了研究，并观察到一个时间和剂量依赖性增加丙二醛。此外，通过使用荧光探针，2 '，7'-二氯荧光素二乙酸酯，发现蓝藻提取物能够以剂量和时间依赖性的方式增强细胞内活性氧（ROS）的产生。此外，去铁胺，一个特定的铁螯合剂，可以显着减少LDH泄漏和活性氧的产生所造成的蓝藻提取物处理。因此，这些研究结果提供了实验证据，氧化损伤参与蓝藻提取物诱导的肝毒性。对这一机制的认识有助于微囊藻毒素和蓝藻污染的防治。(C)北京：科学出版社.
Contamination of water by cyanobacteria (blue-green algae) is a serious health problem around the world, largely due to the toxic effects of microcystins, a group of potent hepatotoxins. However, the mechanisms responsible for the cytotoxicity of microcystins have not been fully elucidated. In the present study, oxidative damage caused by lyophilized freshwater cyanobacteria extract was evaluated on primary cultured rat hepatocytes. A time-and dose-dependent increase of lactate dehydrogenase (LDH) leakage was observed in hepatocytes treated with cyanobacteria extract. Lipid peroxidation, a main manifestation of oxidative damage, was also studied and a time-and dose-dependent increase in malondiadehyde was observed. In addition, by using a fluorescent probe, 2',7'-dichlorofluorescein diacetate, it was found that cyanobacteria extract was able to enhance intracellular production of reactive oxygen species (ROS) in a dose-and time-dependent manner. Moreover, desferrioxamine, a specific iron chelator, could significantly decrease LDH leakage and ROS production caused by cyanobacteria extract treatment. These findings thus provide experimental evidence that oxidative damage is involved in cyanobacteria extract-induced hepatotoxicity. The understanding of this mechanism is believed to be beneficial to the prevention and control of the toxicity of microcystin and cyanobacteria contamination. (C) 1998 Academic Press.