mRNA localization studies suggest that murine FGF-5 plays a role in gastrulation.
mRNA localization studies suggest that murine FGF-5 plays a role in gastrulation.
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发表时间：
1991-06
期刊：
影响因子：
4.6
通讯作者：
J. Hébert;M. Boyle;G. Martin
中科院分区：
文献类型：
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作者：
J. Hébert;M. Boyle;G. Martin
在小鼠原肠胚形成期间，多能胚胎外胚层细胞形成三个初级胚层，外胚层、中胚层和内胚层。对这些过程的机制知之甚少，但来自两栖动物先前研究以及哺乳动物表达研究的证据表明，成纤维细胞生长因子（FGF）家族的信号分子可能在原肠胚形成中发挥作用。为了确定这是否可能是FGF-5在小鼠胚胎中的情况，我们进行了RNA原位杂交研究，以确定何时何地在早期着床后胚胎的FGF-5基因的表达。我们选择研究FGF基因家族的这一特定成员，因为我们先前观察到其在畸胎瘤细胞聚集体培养物中的表达模式与FGF-5在体内原肠胚形成中起作用的提议一致。本文报道的结果表明，就在原肠胚形成之前，多能胚胎外胚层中的Fgf-5表达急剧增加，仅限于原肠胚形成期间形成三个初级种系的细胞，并且一旦形成，在胚胎中的任何细胞中都检测不到。初级种系几乎完成。基于这种挑衅性的表达模式，并在光是已知的功能在体外的FGF家族的其他成员，我们假设，在小鼠胚胎中的FGF-5的功能，以自分泌的方式来刺激的细胞，有助于胚胎胚层的流动性，或使他们有能力响应其他诱导或位置信号。
During gastrulation in the mouse, the pluripotent embryonic ectoderm cells form the three primary germ layers, ectoderm, mesoderm and endoderm. Little is known about the mechanisms responsible for these processes, but evidence from previous studies in amphibians, as well as expression studies in mammals, suggest that signalling molecules of the Fibroblast Growth Factor (FGF) family may play a role in gastrulation. To determine whether this might be the case for FGF-5 in the mouse embryo, we carried out RNA in situ hybridization studies to determine when and where in the early postimplantation embryo the Fgf-5 gene is expressed. We chose to study this particular member of the FGF gene family because we had previously observed that its pattern of expression in cultures of teratocarcinoma cell aggregates is consistent with the proposal that Fgf-5 plays a role in gastrulation in vivo. The results reported here show that Fgf-5 expression increases dramatically in the pluripotent embryonic ectoderm just prior to gastrulation, is restricted to the cells forming the three primary germ layers during gastrulation, and is not detectable in any cells in the embryo once formation of the primary germ layers is virtually complete. Based on this provocative expression pattern and in light of what is known about the functions in vitro of other members of the FGF family, we hypothesize that in the mouse embryo Fgf-5 functions in an autocrine manner to stimulate the mobility of the cells that contribute to the embryonic germ layers or to render them competent to respond to other inductive or positional signals.