Domain III of the T. thermophilus 23S rRNA folds independently to a near-native state
Domain III of the T. thermophilus 23S rRNA folds independently to a near-native state
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DOI：
10.1261/rna.030692.111
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发表时间：
2012-04-01
期刊：
影响因子：
4.5
通讯作者：
Williams, Loren Dean
中科院分区：
文献类型：
--
作者：
Athavale, Shreyas S.;Gossett, J. Jared;Williams, Loren Dean
核糖体大亚基（LSU）的三维结构揭示了一个单一的形态元素，虽然23 S rRNA包含在6个二级结构域。基于地图的域间和域内的相互作用和发展的进化途径，我们假设域III是一个真正独立的结构域的LSU。结构域III主要通过结构域内相互作用稳定，结构域间相互作用的干扰可忽略不计，并且不被核糖体蛋白或其他rRNA穿透。我们使用SHAPE（通过引物延伸分析的选择性2 '-羟基酰化），在不存在和存在镁的情况下，探测了结构域III rRNA单独的结构和包含在完整的23 S rRNA中时的结构。综合结果支持的假设，域III单独折叠到一个接近天然状态的二级结构，域内三级相互作用，和域间的相互作用是独立的，它是否嵌入在完整的23 S rRNA或LSU内。提出的数据支持以前的建议，结构域III被添加到核糖体进化相对较晚。
The three-dimensional structure of the ribosomal large subunit (LSU) reveals a single morphological element, although the 23S rRNA is contained in six secondary structure domains. Based upon maps of inter-and intra-domain interactions and proposed evolutionary pathways of development, we hypothesize that Domain III is a truly independent structural domain of the LSU. Domain III is primarily stabilized by intra-domain interactions, negligibly perturbed by inter-domain interactions, and is not penetrated by ribosomal proteins or other rRNA. We have probed the structure of Domain III rRNA alone and when contained within the intact 23S rRNA using SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension), in the absence and presence of magnesium. The combined results support the hypothesis that Domain III alone folds to a near-native state with secondary structure, intra-domain tertiary interactions, and inter-domain interactions that are independent of whether or not it is embedded in the intact 23S rRNA or within the LSU. The data presented support previous suggestions that Domain III was added relatively late in ribosomal evolution.