Detection of an isoform of α1-antitrypsin in serum samples from foals with gastric ulcers
Detection of an isoform of α1-antitrypsin in serum samples from foals with gastric ulcers
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胃溃疡小马驹血清样本中α1-抗胰蛋白酶亚型的检测
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
H. Yokota
中科院分区：
文献类型：
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作者：
S. Taharaguchi;A. Nagano;K. Okai;T. Miyasho;M. Kuwano;H. Taniyama;H. Yokota
本研究的目的是寻找小马驹胃溃疡的血清指标。应用血清蛋白双向电泳法，在胃镜检查发现胃溃疡的马驹样本中检测到三个明显的斑点。其中一个出现频率较高，经胰酶部分消化和随后的纳米电喷雾电离-串联质谱仪(NanESI-MS/MS)分析鉴定为α-1-抗胰蛋白酶。用抗人α1-抗胰蛋白酶的抗体进行Western印迹分析，发现血清中至少有两条相对分子质量为58 kDa和55 kDa的条带。在47份患胃溃疡的马驹血清中有44份检测到55 kDa条带，而在22份健康马驹的血清样本中只有3份检测到55 kDa条带。
The objective of this study was to find serum indicators of gastric ulcers in foals. By using two-dimensional electrophoresis of serum proteins, three distinct spots were detected in samples from foals with gastric ulcers detected endoscopically. One of them appeared with high frequency and was identified by partial digestion with trypsin and subsequent nano-electrospray ionisation-tandem mass spectrometry (nanoesi-ms/ms) analysis as an α1-antitrypsin. Western blot analysis, using an antibody against human α1-antitrypsin, revealed at least two bands, of molecular weight 58 kDa and 55 kDa, in the sera. The 55 kDa band was detected in 44 of 47 serum samples from foals with gastric ulcers, but in only three of 22 serum samples from healthy foals.