Three-dimensional structure of gap junctions in fragmented plasma membranes from rat liver.
Three-dimensional structure of gap junctions in fragmented plasma membranes from rat liver.
复制标题
大鼠肝脏碎片质膜间隙连接的三维结构。
DOI：
10.1016/s0006-3495(88)82936-9
复制
发表时间：
1988
影响因子：
3.4
通讯作者：
Unwin,N
中科院分区：
文献类型：
--
作者：
Sikerwar,SS;Unwin,N
通过轻微机械破坏大鼠肝脏质膜获得形成广泛六角形晶体片（晶胞尺寸，a=89A）的间隙连接。通过负染色电子显微镜和傅立叶图像处理对这些片材进行三维分析。晶体学对称性显示近似于两侧平面组 p622，表明片材由两个等效、相对的膜组件组成。这些接近天然的连接中的连接子结构与去污剂提取的连接中发现的连接子结构基本相同，亚基与中心六重轴相切且几乎垂直于膜平面排列。
Gap junctions forming extensive hexagonal crystalline sheets (unit cell dimension, a=89 A) were obtained by mild mechanical disruption of plasma membranes from rat liver. The sheets were analysed in three dimensions by negative stain electron microscopy and Fourier image processing. The crystallographic symmetry was shown to approximate to the two-sided plane group p622, indicating that the sheets are composed of two equivalent, oppositely-facing membrane assemblies. The structure of the connexon in these near-to-native junctions is essentially the same as that found in detergent-extracted junctions, the subunits appearing slightly tilted tangential to the central six-fold axis and aligned almost perpendicular to the membrane plane.