Novel Aspects of the Acid Response Network of E. coli K-12 Are Revealed by a Study of Transcriptional Dynamics
Novel Aspects of the Acid Response Network of E. coli K-12 Are Revealed by a Study of Transcriptional Dynamics
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DOI：
10.1016/j.jmb.2010.06.054
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发表时间：
2010-09-03
影响因子：
5.6
通讯作者：
Lund, Peter A.
中科院分区：
文献类型：
--
作者：
Burton, Neil A.;Johnson, Matthew D.;Lund, Peter A.
了解基因调控及其适应性意义不仅需要详细了解引起基因表达变化的单个分子相互作用，还需要全面了解完整的遗传网络及其组件相互作用的方式。越来越多地，这样的研究正在使用发光或荧光报告蛋白，使监测基因表达动态的真实的时间，特别是在表达的变化。我们在这里表明，这种方法是有效的解剖的AR 2或GAD网络的大肠杆菌K-12的pH值的变化，这是在大肠杆菌中已知的最复杂的网络之一的响应。杆菌除了证实先前研究揭示的几种调控相互作用外，这种方法还确定了该系统中的新组分，这些组分导致pH值下降后基因表达的复杂动态，包括涉及YdeO激活蛋白的自动调控环和PhoP蛋白的新作用。(C)2010爱思唯尔有限公司版权所有。
Understanding gene regulation and its adaptive significance requires not only a detailed knowledge of individual molecular interactions that give rise to changes in gene expression but also an overview of complete genetic networks and the ways in which components within them interact. Increasingly, such studies are being done using luminescent or fluorescent reporter proteins that enable monitoring of gene expression dynamics in real time, particularly during changes in expression. We show here that such an approach is valid for dissecting the responses of the AR2 or GAD network of Escherichia coli K-12 to changes in pH, which is one of the most complex networks known in E. coli. In addition to confirming several regulatory interactions that have been revealed by previous studies, this approach has identified new components in this system that lead to complex dynamics of gene expression following a drop in pH, including an auto-regulatory loop involving the YdeO activator protein and novel roles for the PhoP protein. (C) 2010 Elsevier Ltd. All rights reserved.