Low amplitude rhythmic contraction frequency in human detrusor strips correlates with phasic intravesical pressure waves.
Low amplitude rhythmic contraction frequency in human detrusor strips correlates with phasic intravesical pressure waves.
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DOI：
10.1007/s00345-016-1994-0
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发表时间：
2017-08
影响因子：
3.4
通讯作者：
Klausner AP
中科院分区：
文献类型：
--
作者：
Colhoun AF;Speich JE;Cooley LF;Bell ED 3rd;Barbee RW;Guruli G;Ratz PH;Klausner AP
低幅度节律性收缩(LARC)发生在逼尿肌平滑肌，可能在膀胱过度活动(OAB)和逼尿肌过度活动(DO)等储存障碍中发挥作用。本研究的目的是确定体外从人膀胱组织中识别的LARC频率是否与体内LARC频率相关，体内LARC频率在尿动力学(UD)过程中被可视化为相性膀胱内压(Pves)波。在IRB批准后，从患者那里获得新鲜的人膀胱条。LARC在低张力(1t；2g)下用组织条记录，并通过快速傅立叶变换(FFT)分析以识别LARC信号频率。回顾盲法UD轨迹，以寻找充盈期间PVES轨迹上LARC的征象，并通过FFT进行分析。10 0%的组织条(n=9)有不同的LARC频率，平均频率为1.97±0.47次/分钟(33±8 MHz)。在回顾的100项连续的UD研究中，有35项视觉上显示出相位性的PVES波。在12/35(34%)中，真实的PVES信号与腹部活动无关。UD LARC频率为2.34±0.36次/分钟(39±6 MHz)，与组织LARC频率相近(p=0.50)。大多数(83%)有LARC信号的UD患者也表现出逼尿肌过度活动。在UD过程中，一组患者显示了具有明显节律频率的相性pVES波，该频率类似于体外定量的人膀胱组织条中的LARC频率。这项技术的进一步改进可能有助于识别患有LARC介导的储存障碍的个体亚群。
Low amplitude rhythmic contractions (LARC) occur in detrusor smooth muscle and may play a role in storage disorders such as overactive bladder (OAB) and detrusor overactivity (DO). The purpose of this study was to determine whether LARC frequencies identified in vitro from strips of human urinary bladder tissue correlate with in vivo LARC frequencies, visualized as phasic intravesical pressure (pves) waves during urodynamics (UD). After IRB approval, fresh strips of human urinary bladder were obtained from patients. LARC was recorded with tissue strips at low tension (<2g) and analyzed by fast Fourier transform (FFT) to identify LARC signal frequencies. Blinded UD tracings were retrospectively reviewed for signs of LARC on the pves tracing during filling and were analyzed via FFT. Distinct LARC frequencies were identified in 100% of tissue strips (n=9) obtained with a mean frequency of 1.97±0.47 cycles/min (33±8 mHz). Out of 100 consecutive UD studies reviewed, 35 visually displayed phasic pves waves. In 12/35 (34%), real pves signals were present that were independent of abdominal activity. Average UD LARC frequency was 2.34±0.36 cycles/min (39±6 mHz) which was similar to tissue LARC frequencies (p=0.50). A majority (83%) of the UD cohort with LARC signals also demonstrated detrusor overactivity. During UD, a subset of patients displayed phasic pves waves with a distinct rhythmic frequency similar to the in vitro LARC frequency quantified in human urinary bladder tissue strips. Further refinements of this technique may help identify subsets of individuals with LARC–mediated storage disorders.