Comparison of the function of L- and D-proline as compatible solute inEscherichia coli K-12 under high osmolarity
Comparison of the function of L- and D-proline as compatible solute inEscherichia coli K-12 under high osmolarity
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DOI：
10.1007/bf03175217
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发表时间：
2007-06
影响因子：
3
通讯作者：
H. Sasaki;Ai Takaki;A. Oshima;A. Ishida;S. Nagata
中科院分区：
文献类型：
--
作者：
H. Sasaki;Ai Takaki;A. Oshima;A. Ishida;S. Nagata
本文比较了L-脯氨酸和D-脯氨酸在高渗条件下作为大肠杆菌K-12细胞相容性溶质的作用。E.在Davis基本培养基中，0.5 M和1 M NaCl抑制了coliK-12，但通过加入L-脯氨酸可使其恢复。葡萄糖的吸收随外源NaCl浓度的增加而降低，但L-脯氨酸的加入促进了葡萄糖的吸收。另一方面，D-脯氨酸的加入虽然在细胞中积累，但没有表现出相容性溶质的作用。对E. coliproline转运蛋白突变体中，在PutP和ProP两个位点上，脯氨酸转运蛋白对D-脯氨酸的亲和力存在差异，推测D-脯氨酸作为相容性溶质在细胞中的功能紊乱是由其结构特点引起的。
We compared the function of L- and D-proline as compatible solute inEscherichia coliK-12 cells under high osmolarity. Growth ofE. coliK-12 in a Davis minimal medium was inhibited at 0.5 M and 1 M NaCl, but it was recovered by the addition of L-proline. Glucose uptake was reduced with increase of external NaCl concentrations, but it was improved by the addition of L-proline. On the other hand, the addition of D-proline did not show the role of compatible solute although accumulated in cells. On the analysis ofE. coliproline transporter mutants, difference of the affinity of proline transporters for D-proline was observed at PutP and ProP. These results presumed that the functional disorder of D-proline as compatible solute was caused by its structural feature in cells.