STUDIES OF METABOLIC CHANGES IN CELL-CULTURES INFECTED WITH 4 SEROTYPES OF DENGUE FEVER VIRUSES BY FREQUENCY-PULSED ELECTRON-CAPTURE GAS-LIQUID-CHROMATOGRAPHY
STUDIES OF METABOLIC CHANGES IN CELL-CULTURES INFECTED WITH 4 SEROTYPES OF DENGUE FEVER VIRUSES BY FREQUENCY-PULSED ELECTRON-CAPTURE GAS-LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0378-4347(00)85094-x
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
WYCOFF, BJ
中科院分区：
文献类型：
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作者：
BROOKS, JB;KUNO, G;WYCOFF, BJ
用4种血清型的登革病毒感染猴肾细胞培养物，并提取细胞培养物的上清液中的胺、醇、羧酸和羟基酸。然后通过频率脉冲电子捕获气液色谱法（FPEC-GLC）分析衍生化提取物。羟基酸的FPEC-GLC图谱显示不同血清型的峰不同，并且FPEC-GLC羧酸图谱与对照培养基不同。当使用相同批次的培养基时，这些差异是可重现的。由于生长培养基中使用的胎牛血清不同，因此对照培养基批次之间的图谱存在差异。因此，需要相同批次的培养基来重现图谱。从研究中获得的数据表明，FPEC-GLC可用于检测病毒感染引起的细胞代谢变化，这些代谢变化可能有助于检测病毒的遗传差异，如感染细胞代谢的可检测变化所反映的。
Monkey kidney cell cultures were infected with 4 serotypes of dengue viruses, and the supernatant fluids of the cell cultures were extracted for amines, alcohols, carboxylic acids and hydroxy acids. The derivatized extracts were then analyzed by frequency-pulsed electron-capture gas-liquid chromatography (FPEC-GLC). FPEC-GLC profiles of the hydroxy acids showed peaks that were different for differnt serotypes and the FPEC-GLC carboxylic acid profiles differed from the control medium. These differences were reproducible when the same lot of medium was used. There were differences in profiles between lots of control media due apparently to different fetal bovine sera used in the growth medium. Thus, the same lot of medium was necessary to reproduce profiles. The data obtained from the study indicate the FPEC-GLC can be used to detect changes in cellular metabolism caused by viral infection, and that these metabolic changes might be useful for detection of genetic differences in viruses as reflected by detectable changes in the metabolism of the infected cell.