Physicochemical, catalytic, and immunochemical properties of fumarases crystallized separately from mitochondrial and cytosolic fractions of rat liver.
Physicochemical, catalytic, and immunochemical properties of fumarases crystallized separately from mitochondrial and cytosolic fractions of rat liver.
复制标题
从大鼠肝脏的线粒体和胞质部分中分别结晶的延胡索酸酶的物理化学、催化和免疫化学特性。
DOI：
10.1093/oxfordjournals.jbchem.a133394
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发表时间：
1981
影响因子：
2.7
通讯作者：
S. Tuboi
中科院分区：
文献类型：
--
作者：
K. Kobayashi;T. Yamanishi;S. Tuboi
材料和方法在整个实验中使用体重200-250 g的雄性Wistar品系大鼠。亚细胞分离-从通过头部打击使大鼠失去知觉并通过颈部切口流血的的大鼠中迅速取出肝脏。收集的肝脏用5体积（v/w）的含有0.01 M磷酸钾缓冲液（pH 7.3）的0.25 M蔗糖通过使用Potter-Elvehjem
MATERIALS AND METHODSRats-Male Wistar strain rats weighing 200-250 g were used throughout the experiment. The rats were fed on Funahashi laboratory chow ad libitum.Subcellular Fractionation-The livers were removed quickly from rats which had been made insensible by means of a blow on the head and bled through a cervical incision. The collected livers were homogenized with 5 volumes (v/w) of 0.25 M sucrose containing 0.01 M potassium phos phate buffer (pH 7.3) by using a Potter-Elvehjem