Mass Spectra of Bile Acids
Mass Spectra of Bile Acids
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胆汁酸的质谱
DOI：
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发表时间：
1971
期刊：
影响因子：
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通讯作者：
R. Ryhage
中科院分区：
文献类型：
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作者：
J. Sjövall;P. Eneroth;R. Ryhage
在过去的十年里，质谱学已经发展成为胆汁酸分析和结构确定的最有价值的技术之一。这一发展的一个非常重要的原因是建造了能够以高灵敏度水平处理复杂生物混合物的气相色谱-质谱仪。由于生物化学家和临床化学家主要对生物材料的分析感兴趣，本章的目的是提供有关使用这种仪器组合的信息，即使用气相色谱仪作为进气系统或使用质谱仪作为气相色谱检测器。重点放在实际考虑和质谱图与结构之间的相关性上，而不是放在光谱的机械解释上。关于后一个方面的细节，读者可以参考Budzikiewicz，DJerassi和Williams(1，2)的书。
In the last ten years mass spectrometry has grown to become one of the most valuable techniques for analysis and structure determination of bile acids. A very important reason for this development is the construction of combined gas chromatography—mass spectrometry instruments capable of dealing with complex biological mixtures at a high sensitivity level. Since the biochemist and clinical chemist are interested mainly in the analysis of biological materials, the aim of this chapter is to provide information on the use of this instrument combination, i.e., the use of a gas chromatograph as an inlet system or the use of a mass spectrometer as a gas chromatographic detector. Emphasis has been put on practical considerations and on correlations between mass spectra and structure rather than on mechanistic interpretations of the spectra. For details on the latter aspect the reader is referred to the books by Budzikiewicz, Djerassi, and Williams (1, 2).