THE EXPRESSION OF THE IMMEDIATE-EARLY GENES C-FOS, EGR-1 AND C-JUN IN THE ACCESSORY OLFACTORY-BULB DURING THE FORMATION OF AN OLFACTORY MEMORY IN MICE
THE EXPRESSION OF THE IMMEDIATE-EARLY GENES C-FOS, EGR-1 AND C-JUN IN THE ACCESSORY OLFACTORY-BULB DURING THE FORMATION OF AN OLFACTORY MEMORY IN MICE
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DOI：
10.1016/0306-4522(92)90095-j
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发表时间：
1992-07-01
期刊：
影响因子：
3.3
通讯作者：
KEVERNE, EB
中科院分区：
文献类型：
--
作者：
BRENNAN, PA;HANCOCK, D;KEVERNE, EB
雌鼠对与之交配的雄鼠的信息素形成记忆。有人提出，这种记忆背后的突触变化的网站是副嗅球，在第一级的副嗅觉系统。在这项研究中，我们已经检查了即时早期基因c-fos，c-jun和c-jun-1的表达在二尖瓣和颗粒细胞的副嗅球交配后立即，在记忆形成的时期。在记忆形成期间，表达c-fos的颗粒细胞核的数量以及表达c-fos-1的颗粒细胞和二尖瓣细胞核的数量出现短暂增加。c-jun的表达在此期间没有观察到变化。表达c-fos和fos-1的细胞数量的增加需要交配和信息素暴露的关联，这也是记忆形成所需的条件。在没有交配的情况下，将药物荷包牡丹碱注入副嗅球后，还观察到表达c-fos和c-fos-1的二尖瓣和颗粒细胞核数量的大量增加。这些结果表明，c-fos和c-fos-1在副嗅球中的表达与形成雄性信息素嗅觉记忆所需的条件有关。
Female mice form a memory for the pheromones of the male with which they mate. It has been proposed that the site of the synaptic changes underlying this memory is the accessory olfactory bulb, at the first level of the accessory olfactory system. In this study we have examined the expression of the immediate-early genes c-fos, c-jun and egr-1 in the mitral and granule cells of the accessory olfactory bulb immediately after mating, during the period of memory formation. Transient increases were seen in the number of granule cell nuclei expressing c-fos and the number of granule and mitral cell nuclei expressing egr-1, during the period of memory formation. No changes were observed in the expression of c-jun during this period. The increase in the number of cells expressing c-fos and egr-1 required the association of mating and pheromonal exposure, conditions also required for memory formation. Large increases in the number of mitral and granule cell nuclei expressing c-fos and egr-1 were also observed following the infusion of the drug bicuculline into the accessory olfactory bulb in the absence of mating. This procedure has previously been shown to result in the formation of a nonspecific memory for male pheromones.These results associate the expression of c-fos and egr-1 in the accessory olfactory bulb with the conditions required for the formation of an olfactory memory for male pheromones.