Preparation of poly(lactic acid)/chitosan nanoparticles for anti-HIV drug delivery applications
Preparation of poly(lactic acid)/chitosan nanoparticles for anti-HIV drug delivery applications
复制标题
DOI：
10.1016/j.carbpol.2009.12.040
复制
发表时间：
2010-05-05
影响因子：
11.2
通讯作者：
Jayakumar, R.
中科院分区：
文献类型：
--
作者：
Dev, Ashish;Binulal, N. S.;Jayakumar, R.
采用乳液法制备聚乳酸(PLA)/壳聚糖（CS）纳米颗粒，用于抗hiv药物的递送。采用DLS、SEM和FTIR对制备的聚乳酸/CS纳米颗粒进行了表征。将亲水性抗逆转录病毒药物拉米夫定装入PLA/CS纳米颗粒中。采用紫外分光光度计研究了载药聚乳酸/聚苯乙烯纳米颗粒的包封效率和体外释药行为。此外，采用MTT法研究了PLA/CS纳米颗粒的细胞毒性。体外药物释放研究表明，在酸性pH下药物释放速度比在碱性pH下低，这可能是由于高分子纳米颗粒中存在的H离子和阳离子基团之间的排斥作用。药物释放率发现在6%载药制剂比6%载药制剂更高。这些结果表明PLA/CS纳米颗粒是一种很有前途的抗hiv药物控制递送载体体系。(C) 2010 Elsevier Ltd.版权所有。
Poly(lactic acid) (PLA)/chitosan (CS) nanoparticles were prepared by emulsion method for anti-HIV drug delivery applications. The prepared PLA/CS nanoparticles were characterized using DLS, SEM, and FTIR. The hydrophilic antiretroviral drug Lamivudine was loaded into PLA/CS nanoparticles. The encapsulation efficiency and in-vitro drug release behaviour of drug loaded PLA/CS nanoparticles were studied using UV spectrophotometer. In addition, the cytotoxicity of the PLA/CS nanoparticles using MTT assay was also studied. The in-vitro drug release studies showed that drug release rate was lower in the acidic pH when compared to alkaline pH. This may due to repulsion between H ions and cationic groups present in the polymeric nanoparticles. Drug release rate was found to be higher in the 6% drug loaded formulation when compared to 6% drug loaded formulation. These results indicated that the PLA/CS nanoparticles are a promising carrier system for controlled delivery of anti-HIV drugs. (C) 2010 Elsevier Ltd. All rights reserved.