Hepatoprotective potential of isoquercitrin against type 2 diabetes-induced hepatic injury in rats.
Hepatoprotective potential of isoquercitrin against type 2 diabetes-induced hepatic injury in rats.
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DOI：
10.18632/oncotarget.21074
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发表时间：
2017-11-24
期刊：
影响因子：
--
通讯作者：
Lai WM
中科院分区：
文献类型：
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作者：
Huang XL;He Y;Ji LL;Wang KY;Wang YL;Chen DF;Geng Y;OuYang P;Lai WM
非酒精性脂肪肝是2型糖尿病的主要并发症。异槲皮苷用于抗糖尿病治疗，但对肝功能和肝细胞的影响尚不清楚。本研究的目的是探讨异槲皮苷对 T2DM 所致大鼠肝损伤的影响。口服异槲皮苷（10 mg/kg/d、30 mg/kg/d）、磷酸西他列汀（10 mg/kg/d），连续21天。 10 mg/kg/d 和 30 mg/kg/d 的异槲皮苷给药显示出剂量依赖性。与阴性对照（生理盐水治疗）相比，给予异槲皮苷（30 mg/kg/d）和磷酸西格列汀（10 mg/kg/d）的大鼠可改善临床症状、FBG和糖耐量，降低血清ALT、AST和IR，但升高TP、Alb、SOD、GSH、MDA、HDL-C、INS和GLP-1。组织学上，各组大鼠肝组织基本正常，朗格汉斯、变性、坏死和凋亡均明显减少。相反，肝细胞显示出再生。这两组的PKA、AKT、PKCa、InsR和PI3K mRNA表达显着增加，而DPP-IV mRNA水平降低。这些结果表明异槲皮苷治疗可预防 T2DM 引起的肝损伤。
Non-alcoholic fatty liver disease is a main complication of type 2 diabetes. Isoquercitrin are employed for antidiabetic therapies, but the effects on liver function and the hepatocytes are unclear. The aim of this study was to investigate the effects of isoquercitrin on the T2DM-induced hepatic injury in rats. Isoquercitrin (10 mg/kg/d, 30 mg/kg/d), sitagliptin phosphate (10 mg/kg/d) was given orally for 21 days. The administration of isoquercitrin at 10 mg/kg/d and 30 mg/kg/d showed a dose dependent. Compare to the negative control (treated with saline), rats medicated with isoquercitrin (30 mg/kg/d) and sitagliptin phosphate (10 mg/kg/d) improved the clinical symptoms, FBG and glucose tolerance, reduced serum ALT, AST and IR, but increased TP, Alb, SOD, GSH, MDA, HDL-C, INS and GLP-1. On histology, Rats of these to groups presented nearly normal liver tissue and Langerhans, degeneration, necrosis and apoptosis were markedly reduced. Instead, hepatocytes showed regenerate. These two groups also showed significant increase in mRNA expression of PKA, AKT, PKCa, InsR and PI3K, and a decrease in DPP-IV mRNA level. These results indicated that treatment with isoquercitrin protects against hepatic injury by T2DM.