THE TPR-MET ONCOGENIC REARRANGEMENT IS PRESENT AND EXPRESSED IN HUMAN GASTRIC-CARCINOMA AND PRECURSOR LESIONS
THE TPR-MET ONCOGENIC REARRANGEMENT IS PRESENT AND EXPRESSED IN HUMAN GASTRIC-CARCINOMA AND PRECURSOR LESIONS
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DOI：
10.1073/pnas.88.11.4892
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发表时间：
1991-06-01
影响因子：
11.1
通讯作者：
WOGAN, GN
中科院分区：
文献类型：
--
作者：
SOMAN, NR;CORREA, P;WOGAN, GN
TPR-MET致癌重排最初是在体外转化的人骨肉瘤细胞系中观察到的。最近，我们使用基于转录本聚合酶链式反应扩增的高灵敏度方法，在源自非造血来源的人类肿瘤的几种细胞系中检测到这种重排的表达水平非常低。我们在此报告来自人胃肿瘤的细胞系和 22 个显示癌症或先兆病变的人胃粘膜活检样本中 TPR-MET 表达的分析结果。重排的 RNA 在所有四种细胞系以及 22 名患者中 12 名的活检样本中都有表达。 TPR-MET RNA 在伴有腺颈细胞增生的浅表性胃炎病变中过度表达表明该癌基因可能参与胃肿瘤发生的早期阶段。对胃活检样本的 RAS 基因突变分析显示，4 例病例中 Ki- 和 Ha-RAS 基因的密码子 12 区域发生碱基替换，其中包括 2 例前体病变。
The TPR-MET oncogenic rearrangement was originally observed in an in vitro transformed human osteosarcoma cell line. Recently, we detected the expression of this rearrangement at very low levels in several cell lines derived from human tumors of nonhematopoietic origin using a highly sensitive method based on polymerase chain reaction amplification of the transcript. We report here the results of analysis of TPR-MET expression in cell lines derived from human gastric tumors and 22 biopsy samples of human gastric mucosa showing cancer or precursor lesions. The rearranged RNA was expressed in all four cell lines as well as in biopsy samples from 12 of the 22 patients. Overexpression of TPR-MET RNA in superficial gastritis lesions with hyperplasia of glandular neck cells suggests the possible involvement of this oncogene at an early stage of gastric tumorigenesis. Analysis of gastric biopsy samples for RAS gene mutations showed base substitutions occurring in the codon 12 region of Ki- and Ha-RAS genes in four cases, including two precursor lesions.