Effect of cysteine protease of Porphyromonas gingivalis on adhesion molecules in gingival epithelial cells.
Effect of cysteine protease of Porphyromonas gingivalis on adhesion molecules in gingival epithelial cells.
复制标题
牙龈卟啉单胞菌半胱氨酸蛋白酶对牙龈上皮细胞粘附分子的影响。
DOI：
10.1254/jjp.80.75
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
K. Ohura
中科院分区：
文献类型：
--
作者：
Pao‐Li Wang;M. Shinohara;Noboru Murakawa;Masaki Endo;Shigeki Sakata;Makoto Okamura;K. Ohura
本研究检测了牙龈卟啉单胞菌（Porphyromonas gingivalis，P. gingivalis）半胱氨酸蛋白酶对人牙龈上皮细胞细胞间粘附分子-1（ICAM-1）、血管细胞粘附分子-1（VCAM-1）和极晚期抗原-4（VLA-4）的影响。将细胞与或不与牙龈卟啉单胞菌蛋白酶一起孵育48小时。细胞粘附分子的表达水平在12小时时增加，但在18-48小时时下降。在用蛋白酶刺激12小时后，牙龈卟啉单胞菌菌毛与单层细胞的结合被细胞粘附分子的加入有效地抑制，表明菌毛与细胞的结合通过细胞表面粘附分子发生。
We examined the effect of cysteine protease of Porphyromonas gingivalis (P. gingivalis) on cell adhesion molecules including intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) of human gingival epithelial cells. The cells were incubated for 48 hr with or without P. gingivalis protease. Their cell adhesion molecule expression levels were increased at 12 hr, but decreased at 18-48 hr. This result suggests that protease degrades cell adhesion molecules. After the stimulation with protease for 12 hr, P. gingivalis fimbrial binding to a monolayer of the cells was effectively inhibited by the addition of the cell adhesion molecules, suggesting the fimbrial binding to the cells occurred through cell surface adhesion molecules.