Urogenital distribution of a mouse membrane-associated prostaglandin E2 synthase
Urogenital distribution of a mouse membrane-associated prostaglandin E2 synthase
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DOI：
10.1152/ajprenal.0116.2001
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发表时间：
2001-12-01
影响因子：
4.2
通讯作者：
Breyer, MD
中科院分区：
文献类型：
--
作者：
Guan, YF;Zhang, YH;Breyer, MD
PGE（2）在调节肾功能和促进生殖方面起着关键作用。PGE（2）合成的限速酶之一是末端PGE（2）合酶（PGES）。在本研究中，我们报告的功能表达的膜相关的小鼠PGES（mPGES）和它的表达在泌尿生殖系统组织。两个独立的cDNA克隆共享一个相同的开放阅读框架的459 bp和编码一个肽的153个氨基酸，但不同的3 '-非翻译区，被确定。使用从mPGES cDNA转染的细胞制备的微粒体进行的酶活性测定表明，这些cDNA序列编码一种催化PGH（2）转化为PGE（2）的功能蛋白。mPGES在小鼠肾脏、卵巢和膀胱中的组成型表达最高，但在子宫和睾丸中的表达水平也较低。mPGES主要表达于远端小管上皮和髓质集合管。在膀胱和输尿管的移行上皮细胞以及卵巢的初级和次级滤泡中也观察到高表达。总之，mPGES是组成性表达沿着泌尿生殖道，在那里它可能有重要的作用，在正常的生理和疾病。
PGE(2) plays a critical role in regulating renal function and facilitating reproduction. One of the rate-limiting biosynthetic enzymes in PGE(2) synthesis is the terminal PGE(2) synthase (PGES). In the present studies, we report the functional expression of a membrane-associated murine PGES (mPGES) and its expression in urogenital tissues. Two independent cDNA clones sharing an identical open reading frame of 459 bp and encoding a peptide of 153 amino acids, but differing in the 3'-untranslated region, were identified. Assays for enzymatic activity, using microsomes prepared from cells transfected with mPGES cDNA, showed that these cDNA sequences encode a functional protein that catalyzes the conversion of PGH(2) to PGE(2). Constitutive expression of mPGES was highest in the mouse kidney, ovary, and urinary bladder but was also expressed at lower levels in uterus and testis. Renal mPGES expression was predominantly localized to epithelia of distal tubules and medullary collecting ducts. High expression was also seen in transitional epithelial cells of bladder and ureter and in the primary and secondary follicles in the ovary. In conclusion, mPGES is constitutively expressed along the urogenital tract, where it may have important roles in normal physiology and disease.