The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans
The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans
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DOI：
10.1007/s11033-010-0355-0
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发表时间：
2011-04-01
影响因子：
2.8
通讯作者：
Zhu, Xing-Quan
中科院分区：
文献类型：
--
作者：
Liu, Guo-Hua;Lin, Rui-Qing;Zhu, Xing-Quan
线粒体（mt）基因组序列为研究生物群体遗传结构、系统学和遗传学提供了有用的标记。虽然多头带绦虫（Taenia multiceps）、T. hydatigena和T.带形绦虫是反刍动物、猪、狗或猫的常见带绦虫，造成重大经济损失，但尚未有关于其MT基因组的公开研究。对T. multiceps，T. hydatigena和T.在两个重叠片段中扩增带形绦虫，然后测序。T. multiceps，13489 bp。hydatigena，13647 bp。带形肌这三个基因组中的每一个都包含36个基因，其中包括12个蛋白质基因，2个rRNA基因和22个tRNA基因，这与迄今为止研究的所有其他绦虫物种的mt基因组相同。所有基因都以相同的方向转录，并且具有高A和T的核苷酸组成。T全基因组中A+T的含量为71.3%。multiceps，T.棘球蚴感染率为73.0%;带形肌AT偏倚对密码子使用模式和蛋白质氨基酸组成都有显著影响。T. multiceps和T. Hydatigena有两个非编码区，而T.带形肌只有一个。基于12个蛋白质编码基因的氨基酸序列的系统发育分析表明，T。multiceps，T. hydatigena和T.带形绦虫与带绦虫属其他成员的亲缘关系更近，这与以前的形态学和分子研究结果一致。本研究测定了三种对动物和人类健康有重要意义的带绦虫的mt基因组全序列，为研究这些动物和人类绦虫寄生虫的系统学、群体遗传学和分子流行病学提供了有用的标记。
Mitochondrial (mt) genome sequences provide useful markers for investigating population genetic structures, systematics and phylogenetics of organisms. Although Taenia multiceps, T. hydatigena, and T. taeniaeformis are common taeniid tapeworms of ruminants, pigs, dogs, or cats, causing significant economic losses, no published study on their mt genomes is available. The complete mt genomes of T. multiceps, T. hydatigena, and T. taeniaeformis were amplified in two overlapping fragments and then sequenced. The sizes of the entire mt genome were 13700 bp for T. multiceps, 13489 bp for T. hydatigena, and 13647 bp for T. taeniaeformis. Each of the three genomes contains 36 genes, consisting of 12 genes for proteins, 2 genes for rRNA, and 22 genes for tRNA, which are the same as the mt genomes of all other cestode species studied to date. All genes are transcribed in the same direction and have a nucleotide composition high in A and T. The contents of A+T of the complete genomes are 71.3% for T. multiceps, 70.8% for T. hydatigena, and 73.0% for T. taeniaeformis. The AT bias had a significant effect on both the codon usage pattern and amino acid composition of proteins. T. multiceps and T. hydatigena had two noncoding regions, but T. taeniaeformis had only one. Phylogenetic analyses based on concatenated amino acid sequences of 12 protein-coding genes revealed that T. multiceps, T. hydatigena, and T. taeniaeformis were more closely related to the other members of the Taenia genus, consistent with results of previous morphological and molecular studies. The present study determined the complete mt genome sequences for three Taenia species of animal and human health significance, providing useful markers for studying the systematics, population genetics, and molecular epidemiology of these cestode parasites of animals and humans.