Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, tissue distribution and excretion studies of Dactylicapnos scandens in rats
Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, tissue distribution and excretion studies of Dactylicapnos scandens in rats
复制标题
液相色谱-串联质谱法在大鼠体内药代动力学、组织分布和排泄研究中的应用
DOI：
10.1016/j.jpba.2012.10.011
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发表时间：
2013-02-23
影响因子：
3.4
通讯作者：
Zeng, Su
中科院分区：
文献类型：
--
作者：
Guo, Changchuan;Jiang, Yan;Zeng, Su
从 Dactylicapnos scandens 中分离出异紫堇啶和原托品的草药成分。本研究旨在开发一种液相色谱-电喷雾电离-串联质谱 (LC-ESI-MS/MS) 方法来定量大鼠血浆和组织中的异紫堇啶和原托品，用于药代动力学、组织分布和排泄研究。生物样品采用乙酸乙酯萃取，选择紫堇碱作为内标（IS）。分析物通过 C-18 柱分离，并通过三重四极杆质谱仪在多反应监测 (MRM) 模式下使用正离子 ESI 进行检测。监测到的 MS/MS 离子跃迁分别为异堇啶的 m/z 342.0 -> 278.9、原托品的 m/z 354.1 -> 188.0 和 IS 的 m/z 370.0 -> 192.0。在异堇啶 10 至 3000 ng/mL 和原托品 10-300 ng/mL 浓度范围内观察到优异的线性。异堇啶和原托品的定量下限 (LLOQ) 均为 10 ng/mL。该方法快速、准确、灵敏度高、选择性高。已成功应用于D. scandens的药代动力学、组织分布和排泄研究。这些D. scandens的临床前数据可为临床参考。 (C) 2012 Elsevier B.V. 保留所有权利。
The herbal ingredients of isocorydine and protopine were isolated from Dactylicapnos scandens. This study was aimed at developing a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method to quantify isocorydine and protopine in rat plasma and tissues for pharmacokinetic, tissue distribution and excretion studies. Biological samples were processed with ethyl acetate extraction, and corydaline was chosen as the internal standard (IS). The analytes were separated by a C-18 column and detected with a triple quadrupole mass spectrometer using positive ion ESI in the multiple reaction monitoring (MRM) mode. The MS/MS ion transitions monitored were m/z 342.0 -> 278.9 for isocorydine, 354.1 -> 188.0 for protopine and 370.0 -> 192.0 for IS, respectively. Excellent linearity was observed over the concentration range between 10 and 3000 ng/mL for isocorydine and 10-300 ng/mL for protopine. The lower limit of quantification (LLOQ) was 10 ng/mL for both isocorydine and protopine. This novel method was rapid, accurate, high sensitive and high selective. It was successfully applied to the pharmacokinetic, tissue distribution and excretion studies of D. scandens. These preclinical data of D. scandens would be useful for the clinical reference. (C) 2012 Elsevier B.V. All rights reserved.