Localization of Lipids and Lipoprotein in Perivascular FGP Cells of Rat Cerebellar Cortex.
Localization of Lipids and Lipoprotein in Perivascular FGP Cells of Rat Cerebellar Cortex.
复制标题
大鼠小脑皮质血管周围 FGP 细胞中脂质和脂蛋白的定位。
DOI：
--
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
M. Mato
中科院分区：
文献类型：
--
作者：
T. Nakazawa;M. Nishikawa;E. Aikawa;M. Mato
在大鼠脑血管周围发现的一种血管周细胞（荧光颗粒周上皮细胞; FGP细胞）含有在细胞质中显示自发荧光的颗粒。本研究采用8周龄~ 30月龄雄性Wistar大鼠小脑皮质，用细胞化学和免疫细胞化学方法，研究了FGP细胞中脂质和低密度脂蛋白（LDL）的定位，以确定FGP细胞的功能。用酶消化法测定了FGP细胞内甘油三酯（TG）、磷脂（PL）和胆固醇酯（CE）的含量。含TG、PL和CE的内吞囊泡位于FGP细胞内，并与颗粒融合。G_1、G_3、G_4和泡沫状G_5颗粒的膜内及膜周含有TG、PL、CE和LDL。G2颗粒的电子密度较G1颗粒低，在其异质基质中含有TG、PL、CE和LDL。脂质似乎以脂蛋白形式存在于颗粒中。结果表明，FGP细胞采取TG，PL和CE的内吞作用，并将它们内化到这些颗粒作为LDL的成分，在中枢神经系统的血管周围空间的清道夫巨噬细胞的作用。
A type of perivascular cell (fluorescent granular perithelial cell; FGP cell) found around the blood vessels of the rat brain contains granules which show auto-fluorescence in the cytoplasm. In this study, the localization of lipids and low-density lipoprotein (LDL) in these FGP cells was investigated cytochemically and immunocytochemically in order to determine the functions of FGP cells.The cerebellar cortexes of male Wistar rats, aged 8 weeks to 30 months, were used. Triglyceride (TG), phospholipid (PL) and cholesteryl ester (CE) were revealed in FGP cells by using enzymic digestive methods. Endocytotic vesicles containing TG, PL and CE were located inside the FGP cells and were fused with granules. G1, G3, G4 and foamy G5 granules had TG, PL, CE and LDL in and around their membranes. G2 granules, with lower electron density than G1 granules, contained TG, PL, CE and LDL in their heterogeneous matrices. Lipids appeared to occur in granules as a lipoprotein. The results suggest that FGP cells take up TG, PL and CE by endocytosis and internalize them into these granules as components of LDL, playing the role of a scavenger macrophage in the perivascular space of the central nervous system.
影响因子：
56.9
作者：
HABERLAND, ME;FONG, D;CHENG, L
通讯作者：
CHENG, L
人类动脉粥样硬化病变的提取物可以修饰低密度脂蛋白，从而增强巨噬细胞的摄取。
影响因子：
5.3
作者：
Hoff,HF;O'Neil,J
通讯作者：
O'Neil,J