Proteome Profiling of 1 to 5 Spiked Circulating Tumor Cells Isolated from Whole Blood Using Immunodensity Enrichment, Laser Capture Microdissection, Nanodroplet Sample Processing, and Ultrasensitive nanoLC-MS.
Proteome Profiling of 1 to 5 Spiked Circulating Tumor Cells Isolated from Whole Blood Using Immunodensity Enrichment, Laser Capture Microdissection, Nanodroplet Sample Processing, and Ultrasensitive nanoLC-MS.
复制标题
DOI：
10.1021/acs.analchem.8b03268
复制
发表时间：
2018-10-16
影响因子：
7.4
通讯作者：
Kelly RT
中科院分区：
文献类型：
--
作者：
Zhu Y;Podolak J;Zhao R;Shukla AK;Moore RJ;Thomas GV;Kelly RT
Proteome profiling of circulating tumor cells (CTCs) can provide crucial insight into disease progression and the role of CTCs in tumor metastasis. We describe an integrated workflow to measure global protein expression in 1–5 spiked CTCs enriched from whole blood by immunodensity gradient centrifugation. Enriched CTCs were purified and collected by laser capture microdissection, prepared using a recently developed nanodroplet-based processing platform (nanoPOTS), and finally analyzed by ultrasensitive nanoLC-MS/MS. The workflow was capable of identifying an average of 164 and 607 protein groups from samples comprising 1 and 5 LNCaP cells, respectively, that were isolated from human whole blood. A panel of prostate cancer-specific proteins were identified and quantified, which was used to differentiate between spiked CTCs and white blood cells.
登录
查看更多内容
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
DOI：
10.1126/science.1235226
发表时间：
2013-09-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Plaks V;Koopman CD;Werb Z
通讯作者：
Werb Z
影响因子：
7
作者：
Li, Siyang;Plouffe, Brian D.;Ivanov, Alexander R.
通讯作者：
Ivanov, Alexander R.
影响因子：
7.4
作者：
Shen, Y;Tolic, N;Smith, RD
通讯作者：
Smith, RD
影响因子：
64.8
作者：
Schwanhaeusser, Bjoern;Busse, Dorothea;Selbach, Matthias
通讯作者：
Selbach, Matthias