CCL21 modulates the migration of NSCL cancer by changing the concentration of intracellular Ca2+.
CCL21 modulates the migration of NSCL cancer by changing the concentration of intracellular Ca2+.
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DOI：
10.3892/or.2011.1528
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发表时间：
2011-11
期刊：
影响因子：
4.2
通讯作者：
Jun Liu;Lei Zhang;Changli Wang
中科院分区：
文献类型：
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作者：
Jun Liu;Lei Zhang;Changli Wang
复发和转移是影响非小细胞肺癌（NSCLC）预后的主要因素。研究表明，多种趋化因子及其受体与NSCLC的进展和转移有关。本研究的目的是研究CCL 21及其受体CCR 7是否在NSCLC侵袭和转移中发挥作用。采用Western blotting、免疫细胞化学和流式细胞术检测CCR 7蛋白在EKVX、HOP-62、NCI-H23和Slu-01四种非小细胞肺癌细胞系中的表达，并进行细胞迁移实验，观察肺癌细胞伪足的形成和迁移能力。用荧光显微镜测量细胞内钙浓度。CCR 7蛋白在EKVX、HOP-62、NCI-H23和Slu-01四种NSCLC细胞系中呈阳性表达。CCL 21刺激后，观察到肺癌细胞明显伪足形成。细胞迁移实验表明，与CCL 21孵育后，通过聚碳酸酯微孔滤膜的EKVX细胞数量也明显增加。经CCL 21孵育后，EKVX细胞内Ca ~（2+）水平明显升高。趋化因子CCL 21通过改变细胞内Ca 2+浓度促进肺癌的迁移。CCL 21-CCR 7轴可能在NSCLC侵袭转移中起重要作用。它也可能成为NSCLC治疗或预防NSCLC复发和转移的潜在靶点。
Recurrence and metastasis are the major factors associated with the poor prognosis of non-small cell lung cancer (NSCLC). It has been shown that multiple chemokines and their receptors are related to the progression and metastasis of NSCLC. The aim of this study was to conduct an investigation into whether CCL21 and its receptor, CCR7, play a role in NSCLC invasion and metastasis. We used Western blotting, immunocytochemistry and flow cytometry to detect CCR7 protein expression in four NSCLC cell lines EKVX, HOP-62, NCI-H23 and Slu-01; and we conducted a cell migration experiment to observe the pseudopodia formation and mobility of the lung cancer cells. The concentration of intracellular calcium was measured by fluorescence microscopy. CCR7 protein was positively expressed in the four NSCLC cell lines EKVX, HOP-62, NCI-H23 and Slu-01. Following CCL21 stimulation, obvious pseudopodia formation of lung cancer cells was observed. The cell migration experiment showed that following incubation with CCL21, the number of EKVX cells which passed through the polycarbonate micro-porous filter membranes also increased to an obvious extent. After CCL21 incubation, the intracellular Ca2+ level of the EKVX cells increased to an obvious extent. Chemokine CCL21 facilitates the migration of lung cancer by changing the concentration of intracellular Ca2+. The CCL21-CCR7 axis may play an important role in NSCLC invasion and metastasis. It may also be a potential target for NSCLC therapy or for prevention of the recurrence and metastasis of NSCLC.