Validation of a blood plasma separation system by biomarker detection
Validation of a blood plasma separation system by biomarker detection
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DOI：
10.1039/b926834k
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Desmulliez, Marc P. Y.
中科院分区：
文献类型：
--
作者：
Kersaudy-Kerhoas, Maiwenn;Kavanagh, Deirdre M.;Desmulliez, Marc P. Y.
研制了一种用于高流速血浆分离的微流控系统。该系统仅使用流体动力将血浆与全血分离。微流控网络具有一系列收缩和分叉，以提高产品产量和纯度。在入口红细胞压积水平高达30%的血液上获得100%的最大纯度效率，流速为2 mL h（-1）。对提取的血浆进行流式细胞术以评价分离效率并评估细胞损伤。本研究的核心目标是从芯片上提取的血浆中检测无细胞DNA。为此，在芯片上提取的血浆中存在的游离DNA上成功地进行了芯片外PCR。在分离后不需要纯化就扩增了管家基因序列（GAPDH），从而显示了血浆样品的高质量。所得数据表明，该系统可以用作总分析系统的初步模块，用于人血浆中的无细胞DNA检测。
A microfluidic system was developed for blood plasma separation at high flow rate. This system uses only hydrodynamic forces to separate plasma from whole blood. The microfluidic network features a series of constrictions and bifurcations to enhance the product yield and purity. A maximum purity efficiency of 100% is obtained on blood with entrance hematocrit level up to 30% with a flow rate of 2 mL h(-1). Flow cytometry was performed on the extracted plasma to evaluate the separation efficiency and to assess cell damage. A core target of this study was the detection of cell-free DNA from the on-chip extracted plasma. To this effect, PCR was successfully carried out off-chip on the cell-free DNA present in the plasma extracted on-chip. A house-keeping gene sequence (GAPDH) was amplified without the need for a purification after the separation, thereby showing the high quality of the plasma sample. The resulting data suggests that the system can be used as a preliminary module of a total analysis system for cell-free DNA detection in human plasma.