Isolation and characterization of mammalian orthoreovirus type 3 from a fecal sample from a wild boar in Japan
Isolation and characterization of mammalian orthoreovirus type 3 from a fecal sample from a wild boar in Japan
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DOI：
10.1007/s00705-021-05053-7
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发表时间：
2021-04-11
影响因子：
2.7
通讯作者：
Nagai, Makoto
中科院分区：
文献类型：
--
作者：
Zhang, Wenjing;Kataoka, Michiyo;Nagai, Makoto
哺乳动物正呼肠孤病毒（MRV）已在各种哺乳动物物种中鉴定，包括人、蝙蝠和猪。然而，从野猪中分离的MRV和全基因组序列尚未报道。在这项研究中，我们分离，测序，并分析了MRV从一个自由生活的野猪在日本使用的猪萨佩罗病毒耐药细胞系N1380。从N1380细胞培养上清液中获得完整和空病毒颗粒，并从完整病毒颗粒中获得完整基因组序列。序列分析表明，该分离株（TY-14）属于MRV 3型，与日本动物园狮子MRV 2分离株（L2、L3和M3基因）和日本人MRV 2分离株（S2基因）有较近的亲缘关系。系统进化树分析表明，TY-14在M1系统进化树中仅与蝙蝠MRV聚为一簇，在M2和S3系统进化树中与多个动物MRV聚为一簇，在L1、S1和S4系统进化树中分别独立分支，表明TY-14与来源不明的病毒有亲缘关系。在M2基因中鉴定了突变事件。这些结果表明，TY-14是通过涉及日本流行的MRV、蝙蝠病毒和其他来源不明的病毒的重配和重组事件产生的。
Mammalian orthoreoviruses (MRVs) have been identified in various mammalian species, including humans, bats, and pigs. However, isolation and complete genome sequences of MRVs from wild boars have not yet been reported. In this study, we isolated, sequenced, and analyzed an MRV from a free-living wild boar in Japan using the porcine-sapelovirus-resistant cell line N1380. Complete and empty virus particles were obtained from the N1380 cell culture supernatants, and complete genome sequences were obtained from complete virus particles. Sequence analysis revealed that the isolated MRV, named TY-14, could be classified as MRV3 and had a close genetic relationship to an MRV2 isolate from a lion in a Japanese zoo (L2, L3, and M3 genes) and a human MRV2 isolate from Japan (S2 gene). Phylogenetic analysis showed that TY-14 clustered only with bat MRVs in the M1 phylogenetic tree but formed a cluster with several animal MRVs in the M2 and S3 phylogenetic trees and branched independently in the L1, S1, and S4 phylogenetic trees, suggesting a genetic relationship to viruses of unknown origin. Recombination events were identified in the M2 gene. These results suggest that TY-14 was generated by reassortment and recombination events involving MRVs circulating in Japan, viruses from bats, and other viruses of unknown origin.