Three-dimensional structure of Fab R19.9, a monoclonal murine antibody specific for the p-azobenzenearsonate group.
Three-dimensional structure of Fab R19.9, a monoclonal murine antibody specific for the p-azobenzenearsonate group.
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Fab R19.9 的三维结构，Fab R19.9 是一种对偶氮苯胂酸基团特异性的单克隆鼠抗体。
DOI：
10.1073/pnas.86.2.607
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发表时间：
1989
影响因子：
11.1
通讯作者：
Poljak,RJ
中科院分区：
文献类型：
--
作者：
Lascombe,MB;Alzari,PM;Boulot,G;Saludjian,P;Tougard,P;Berek,C;Haba,S;Rosen,EM;Nisonoff,A;Poljak,RJ
The crystal structure of Fab R19.9, derived from an anti-p-azobenzenearsonate monoclonal antibody, has been determined and refined to 2.8-A resolution by x-ray crystallographic techniques. Monoclonal antibody R19.9 (IgG2b kappa) shares some idiotopes with a major idiotype (CRIA) associated with A/J anti-p-azobenzenearsonate antibodies. The amino acid sequences of the variable (V) parts of the heavy (VH) and light (VL) polypeptide chains of monoclonal antibody R19.9 were determined through nucleotide sequencing of their mRNAs. The VL region is very similar to that of CRIA-positive anti-p-azobenzenearsonate antibodies as is VH, except for its third complementarity-determining region, which is three amino acids longer; it makes a loop, unique to R19.9, that protrudes into the solvent. A large number of tyrosine residues in the complementarity-determining region of VH and VL, with their side chains pointing towards the solvent, may have an important function in antigen binding.
