Autocrine growth function of human interleukin 1 molecules on ROHA-9, an EBV-transformed human B cell line.
Autocrine growth function of human interleukin 1 molecules on ROHA-9, an EBV-transformed human B cell line.
复制标题
人白介素 1 分子在 EBV 转化的人 B 细胞系 ROHA-9 上的自分泌生长功能。
作者：
G. Scala;G. Morrone;M. Tamburrini;F. Alfinito;C. Pastore;G. D'alessio;S. Venuta
在这项研究中，我们报告的ROHA-9细胞系，IL 1分泌的EBV转化的人B细胞系，表现出自分泌途径的增长。事实上，ROHA-9细胞自发地分泌与组成型分泌的IL 1样分子共纯化的自身调节生长因子。因此，不含其它已知生物活性的单核细胞衍生的人IL 1也以剂量依赖性方式刺激ROHA-9细胞的生长。人重组白细胞介素2、重组IFN-α或IFN-γ和纯化IFN-β在浓度高达1 × 10（3）U/ml时无效。此外，小鼠重组IL-1，HPLC纯化的多集落刺激因子和部分纯化的BCGF制剂在测定对ROHA-9细胞的生长促进活性时无效。此外，兔多克隆抗体和小鼠单克隆抗体的人IL 1分子阻断ROHA-9细胞的生长诱导的自体生长因子和人IL 1。最后，纯化的人IL 1增加了以低细胞浓度接种的ROHA-9细胞的克隆效率，允许分离ROHA-9 MC 3亚克隆，其显示出相似的生长反应特异性并且对人IL 1的促有丝分裂活性特别敏感。
In this study we report that the ROHA-9 cell line, an IL 1-secreting EBV-transformed human B cell line, exhibits an autocrine pathway of growth. In fact, ROHA-9 cells spontaneously secreted an autoregulatory growth factor that co-purified with the constitutively secreted IL 1-like molecules. Accordingly, monocyte-derived human IL 1, free of other known biological activities, also stimulated the growth of ROHA-9 cells in a dose-dependent way. Human recombinant interleukin 2, recombinant IFN-alpha or IFN-gamma and purified IFN-beta were ineffective when used at concentrations up to 1 X 10(3) U/ml. Furthermore, mouse recombinant IL 1, HPLC-purified multi-colony stimulating factor and partially purified preparations of BCGF were ineffective when assayed for growth-promoting activity on ROHA-9 cells. Moreover, a rabbit polyclonal antibody and a mouse monoclonal antibody to human IL 1 molecules blocked the growth of ROHA-9 cells induced by the autologous growth factor and by human IL 1. Lastly, purified human IL 1 increased the clonal efficiency of ROHA-9 cells seeded at a low cell concentration, allowing the isolation of the ROHA-9MC3 subclone, which showed similar growth response specificity and was particularly sensitive to the mitogenic activity of human IL 1.