Isolation and characterization of lac fusions to two nitrogen-regulated promoters.
Isolation and characterization of lac fusions to two nitrogen-regulated promoters.
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两个氮调节启动子的 lac 融合体的分离和表征。
DOI：
10.1007/bf00332750
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Ames,GF
中科院分区：
文献类型：
--
作者：
Stern,MJ;Higgins,CF;Ames,GF
Mud1（Ap，lac，cts）介导的与argTranddhuA（鼠伤寒沙门氏菌中的两个转运操纵子启动子）的融合被分离和表征，以研究这些启动子的调节。使用这些融合，我们表明这些启动子受到氮调节，并且这种效应以及对启动子上突变 indhuA 的反应是在转录水平上。我们利用融合来确定组氨酸转运操纵子不包含任何内部启动子。融合还用于筛选启动子的其他调节模式：发现arg Tr除了氮调节之外还响应碳调节，而dhuA则不然。 argTr启动子包含与cAMP受体蛋白结合位点确定的共有序列具有良好同源性的序列。两种启动子都不响应硫或磷酸盐的调节。
Mud1(Ap,lac,cts)-mediated fusions toargTranddhuA, two transport operon promoters inSalmonella typhimurium, were isolated and characterized in order to investigate the regulation of these promoters. Using these fusions we showed that these promoters are under nitrogen regulation and that this effect, as well as the response to a promoter-up mutation indhuA, is at the transcriptional level. We utilized the fusions to determine that the histidine transport operon does not contain any internal promoters. The fusions were also used to screen the promoters for additional modes of regulation:arg Trwas found to respond to carbon regulation in addition to nitrogen regulation whiledhuAdoes not. TheargTrpromoter contains a sequence with good homology to the consensus sequence determined for the cAMP receptor protein binding site. Neither promoter responds to sulfur or phosphate regulation.