Development of monoclonal antibodies that specifically react with a fish pathogen, Vibrio anguillarum serotype J-O-1
Development of monoclonal antibodies that specifically react with a fish pathogen, Vibrio anguillarum serotype J-O-1
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DOI：
10.2331/fishsci.62.710
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发表时间：
1996-10-01
影响因子：
1.9
通讯作者：
Eguchi, M
中科院分区：
文献类型：
--
作者：
Miyamoto, N;Eguchi, M
用杂交瘤技术制备了5株抗鳗弧菌J-O-1型细胞表面抗原的单抗。其中3株单抗VA19-1、VA19-2和VA19-4仅与J-O-1血清型反应。其余两株单抗VA07-1和VA07-2与其他弧菌有交叉反应。单抗VA19-1、VA19-2和VA19-4可与100℃热处理的鳗弧菌J-O-1细胞反应。这说明这些单抗识别的抗原是脂多糖等稳定的分子。由于淡水鳗弧菌几乎所有的菌株都属于J-O-1血清型，因此，VA19-1、VA19-2和VA19-4单抗可用于自然淡水环境中鳗弧菌的快速鉴定和直接检测。此外，单抗VA19-1、VA19-2和VA19-4与10个丹麦血清型(O1和O10相似)中的O2型鳗弧菌特异反应，而不与Ordalii弧菌反应。因此，这些单抗也可用于区分关系密切的鳗弧菌O2和奥氏弧菌。
Five monoclonal antibodies (MAbs) against cell surface antigens of Vibrio anguillarum serotype J-O-1 were developed by a hybridoma technique. Three of the MAbs, VA19-1, VA19-2, and VA19-4, reacted only with serotype J-O-1. The remaining two MAbs, VA07-1 and VA07-2, showed cross-reaction with some other Vibrio species. MAbs VA19-1, VA19-2, and VA19-4 could react with heat treated (100 degrees C for 1 h) cells of V. anguillarum J-O-1. This indicated that the antigens recognized by these MAbs were stable molecules such as lipopolysaccharide. As almost all strains of V. anguillarum isolated from diseased ayu Plecoglossus altivelis in freshwater belong to serotype J-O-1, MAbs VA19-1, VA19-2, and VA19-4 are useful for rapid identification and direct detection of V. anguillarum J-O-1 in natural freshwater environments. Furthermore, MAbs VA19-1, VA19-2, and VA19-4 specifically reacted with V. anguillarum serotype O2 among ten Danish serotypes (O1 similar to O10) and did not react with V. ordalii. Thus, these MAbs are also useful for distinction between closely related species, V. anguillarum O2 and V. ordalii.