SPATIALLY LOCALIZED H-1-NMR SPECTRA OF METABOLITES IN THE HUMAN-BRAIN
SPATIALLY LOCALIZED H-1-NMR SPECTRA OF METABOLITES IN THE HUMAN-BRAIN
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DOI：
10.1073/pnas.85.6.1821
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发表时间：
1988-03-01
影响因子：
11.1
通讯作者：
SHULMAN, RG
中科院分区：
文献类型：
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作者：
HANSTOCK, CC;ROTHMAN, DL;SHULMAN, RG
使用表面线圈，我们已经从人脑中的代谢物获得了1H核磁共振谱。通过结合深度脉冲和图像选择的在体光谱磁场梯度方法实现定位。在4分钟内从14毫升脑组织获得了总肌酸(3.03ppm)的1H谱，其信噪比为95：1。相对于灰质和白质的肌酸峰，测量到2.02ppm的共振，主要由N-乙酰天冬氨酸组成，而在白质中这一比例较低。测量了白质和灰质N-乙酰天冬氨酸和肌酸的自旋-自旋松弛时间，当两者的肌酸松弛时间相同时，白质的N-乙酰天冬氨酸松弛时间较长。在正常缺氧的大脑中检测到乳酸，三次测量的平均值为.apprxeq。与肌酸加磷酸肌酸峰相比，0.5 mm，假定为10.5 mm。
Using a surface coil, we have obtained 1H NMR spectra from metabolites in the human brain. Localization was achieved by combining depth pulses with image-selected in vivo spectroscopy magnetic field gradient methods. 1H spectra in which total creatine (3.03 ppm) has a signal/noise ratio of 95:1 were obtained in 4 min from 14 ml of brain. A resonance at 2.02 ppm consisting predominantly of N-acetylaspartate was measured relative to the creatine peak in gray and white matter, and the ratio was lower in the white matter. The spin-spin relaxation times of n-acetylaspartate and creatine were measured in white and gray matter and while creatine relaxation times were the same in both, the N-acetylaspartate relaxation time was longer in white matter. Lactate was detected in the normoxic brain and the average of three measurements was .apprxeq. 0.5 mM from comparison with the creatine plus phosphocreatine peak, which was assumed to be 10.5 mM.