Expression profiling of non-aflatoxigenic Aspergillus parasiticus mutants obtained by 5-azacytosine treatment or serial mycelial transfer.
Expression profiling of non-aflatoxigenic Aspergillus parasiticus mutants obtained by 5-azacytosine treatment or serial mycelial transfer.
复制标题
DOI：
10.3390/toxins3080932
复制
发表时间：
2011-08
期刊：
影响因子：
4.2
通讯作者：
Ehrlich KC
中科院分区：
文献类型：
--
作者：
Wilkinson JR;Kale SP;Bhatnagar D;Yu J;Ehrlich KC
黄曲霉毒素是由真菌黄曲霉和寄生曲霉产生的致癌次生代谢物。先前的研究发现，重复的连续菌丝转移或用5-氮胞苷处理的寄生蜂产生的菌落具有蓬松的表型，不能产生黄曲霉毒素。为了了解这些处理如何影响黄曲霉毒素产生和发展相关基因的表达，我们进行了基于表达序列标签（EST）的微阵列检测，以鉴定处理克隆中与野生型相比表达差异的基因。183个基因表达显著失调。其中，与未治疗的对照组相比，38个至少有两倍或更低的表达，只有两个有两倍或更高的表达。最常见的变化是编码膜结合蛋白的基因下调。基于这一结果，我们假设这些处理导致细胞和细胞器膜结构的改变，从而阻止了黄曲霉毒素的正常发育和生物合成。
Aflatoxins are carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Previous studies found that repeated serial mycelial transfer or treatment of A. parasiticus with 5-azacytidine produced colonies with a fluffy phenotype and inability to produce aflatoxins. To understand how these treatments affect expression of genes involved in aflatoxin production and development, we carried out expressed sequence tag (EST)-based microarray assays to identify genes in treated clones that are differentially expressed compared to the wild-type. Expression of 183 genes was significantly dysregulated. Of these, 38 had at least two-fold or lower expression compared to the untreated control and only two had two-fold or higher expression. The most frequent change was downregulation of genes predicted to encode membrane-bound proteins. Based on this result we hypothesize that the treatments cause changes in the structure of cellular and organelle membranes that prevent normal development and aflatoxin biosynthesis.