Evaluation of the LIAISON ANA screen assay for antinuclear antibody testing in autoimmune diseases
Evaluation of the LIAISON ANA screen assay for antinuclear antibody testing in autoimmune diseases
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DOI：
10.1196/annals.1398.046
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发表时间：
2007-01-01
期刊：
影响因子：
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通讯作者：
Musset, L.
中科院分区：
文献类型：
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作者：
Ghillani, P.;Rouquette, A. M.;Musset, L.
在结缔组织疾病和其他病理疾病患者中，抗核抗体(ANA)广泛应用于Hep-2细胞上的免疫荧光检测。我们评价了第一个用于检测ANA的自动化学发光免疫分析(DiaSorin)。这项研究同时在两个实验室进行，检测了327例临床定义为结缔组织疾病的患者样本，273例用于ANA筛查的常规样本，以及300名献血者。337份IIF阳性血清中，联络性ANA筛查阳性268例(79.5%)，IIF阴性263例中240例阴性(91.2%)。消解差异结果后，符合率分别达到94.9%和98.8%。其特异度为99.3%，灵敏度为94%。与其他ANA筛查结果不同，我们观察到了可接受的敏感性和特异性。尽管有Hep-2细胞提取物的存在，但我们未能检测到一些抗体，如抗核仁、抗核膜和抗增殖细胞核抗原。这种自动化分析方法可以快速处理结果，并表现出令人满意的灵敏度，用于检测结缔组织病的主要ANA特异性。
Antinuclear antibodies (ANA) are widely detected by immunofluorescence on HEp-2 cells in patients with connective tissue diseases and other pathological conditions. We evaluated the first-automated chemiluminescence immunoassay for the detection of ANA (LIAISON ANA screen, DiaSorin). This study was carried out simultaneously in two laboratories by testing 327 patient samples with clinically defined connective diseases, 273 routine samples for ANA screening, and 300 blood donors. A total of 268 out of 337 IIF-positive sera were positive with LIAISON ANA screen (79.5% of agreement) and 240 out of 263 IIF-negative sera were negative with LIAISON ANA screen (91.2% of agreement). After resolution of discrepant results, the concordance reached, respectively, 94.9% and 98.8%. The specificity was 99.3% and the sensitivity was 94%. Unlike results obtained by other ANA screening assays, we observed acceptable sensitivity and specificity. Despite the presence of HEp-2 cell extract, we failed to detect some antibodies as antinucleolar, antinuclear envelope, and antiproliferating cell nuclear antigen. This automated assay allows quick process to results and exhibits satisfactory sensitivity for the detection of the main ANA specificities of connective tissue diseases.