Biocompatibility of silk-tropoelastin protein polymers
Biocompatibility of silk-tropoelastin protein polymers
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DOI：
10.1016/j.biomaterials.2014.03.024
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发表时间：
2014-06-01
期刊：
影响因子：
14
通讯作者：
Bao, Shisan
中科院分区：
文献类型：
--
作者：
Liu, Hongjuan;Wise, Steven G.;Bao, Shisan
混合聚合物作为永久植入设备的组件广泛应用于许多关键的医疗条件。含有重组人原弹性蛋白和丝素蛋白的杂化蛋白聚合物在力学和生物学性能方面具有良好的可植入支架的特性。利用萤火虫荧光素酶转基因小鼠模型，实时监测生物材料植入部位IL-1β的产生，观察对材料的急性免疫反应(最长5天)。在术后1、2和3天，真丝/原弹性蛋白植入物的IL-1β水平显著低于单纯真丝植入物。随后，分别于术后10d(10d)和3w(3w)处死9只小鼠，用组织病理学和免疫组织化学方法观察炎性细胞浸润和胶原沉积情况。与仅植入真丝的对照相比，真丝/原弹性蛋白中的炎症细胞总数较少(10d时类似于29%，3W时类似47%)。此外，与仅植入真丝相比，植入真丝/原弹性蛋白植入物的植入细胞较少(10d时类似于42%，3W时类似于63%)。在第10天，丝蛋白/原弹性蛋白也检测到较低的IL-6(类似于52%)和基质金属蛋白酶-2(类似于84%)(促炎)。植入3周后，蚕丝/原弹性蛋白材料与单纯蚕丝相比，新生血管减少(vWF类似43%)，增殖细胞减少(167个类似58%，增殖细胞核抗原类似41%)，巨噬细胞(F4/80类似%)，IL-10(类似47%)和MMP9(类似55%)也降低。综上所述，这些结果表明，原弹性蛋白的加入改善了丝素蛋白已建立的生物相容性，这在这里是唯一被测量为减少异物炎症反应的。皇冠版权所有(C)2014由爱思唯尔有限公司出版。保留所有权利。
Blended polymers are used extensively in many critical medical conditions as components of permanently implanted devices. Hybrid protein polymers containing recombinant human tropoelastin and silk fibroin have favorable characteristics as implantable scaffolds in terms of mechanical and biological properties. A firefly luciferase transgenic mouse model was used to monitor real-time IL-1 beta production localized to the site of biomaterial implantation, to observe the acute immune response (up to 5 days) to these materials. Significantly reduced levels of IL-1 beta were observed in silk/tropoelastin implants compared to control silk only implants at 1, 2 and 3 days post-surgery. Subsequently, mice (n = 9) were euthanized at 10 days (10D) and 3 weeks (3W) post-surgery to assess inflammatory cell infiltration and collagen deposition, using histopathology and immunohistochemistry. Compared to control silk only implants, fewer total inflammatory cells were found in silk/tropoelastin (similar to 29% at 10D and similar to 47% at 3W). Also fewer ingrowth cells (similar to 42% at 10D and similar to 63% at 3W) were observed within the silk/tropoelastin implants compared to silk only. Lower IL-6 ( similar to 52%) and MMP-2 ( similar to 84%) (pro-inflammatory) were also detected for silk/tropoelastin at 10 days. After 3 weeks implantation, reduced neovascularization (vWF similar to 43%), fewer proliferating cells (1(167 similar to 58% and PCNA similar to 41%), macrophages (F4/80 similar to 64%), lower IL-10 (similar to 47%) and MMP-9 (similar to 55%) were also observed in silk/tropoelastin materials compared to silk only. Together, these results suggest that incorporation of tropoelastin improves on the established biocompatibility of silk fibroin, uniquely measured here as a reduced foreign body inflammatory response. Crown Copyright (C) 2014 Published by Elsevier Ltd. All rights reserved.