On chip detection of glial cell-derived neurotrophic factor secreted from dopaminergic cells under magnetic stimulation
On chip detection of glial cell-derived neurotrophic factor secreted from dopaminergic cells under magnetic stimulation
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磁刺激下多巴胺能细胞分泌的胶质细胞源性神经营养因子的芯片检测
DOI：
10.1016/j.bios.2021.113179
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发表时间：
2021
影响因子：
12.6
通讯作者：
Que, Long
中科院分区：
文献类型：
--
作者：
Yang, Renyuan;Boldrey, Joseph;Jiles, David;Schneider, Ian;Que, Long
胶质细胞源性神经营养因子（GDNF）是一种小蛋白质，可有效促进多巴胺能神经元和运动神经元的存活。 GDNF 可以从不同类型的细胞分泌，包括多巴胺能神经细胞系 N27。 N27细胞是一种大鼠多巴胺能神经细胞系，被认为是帕金森病（PD）研究的合适体外模型。对于PD治疗，经颅磁刺激（TMS）这种无创治疗方法显示出有益的临床效果，但其益处的机制尚不清楚。由于 GDNF 是一种有效的神经营养因子，因此评估 N27 细胞的 GDNF 分泌是否会受到磁刺激 (MS) 的影响具有重要价值。然而，目前检测 GDNF 的方法既耗时又昂贵。在本文中，我们首次概述了超灵敏纳米孔薄膜传感器（纳米传感器）对 N27 细胞分泌的 GDNF 的检测。纳米传感器可以轻松检测到低至 2 pg/mL 的 GDNF。此外，我们还发现 MS 可以促进 N27 细胞分泌 GDNF。具体而言，与对照相比，在体外5天后，MS处理下的N27细胞条件培养基中的GDNF浓度显示出统计学上显着的增加，高达2倍。这种纳米传感器与体外 PD 模型 N27 细胞一起提供了一种低成本、易于使用、灵敏的方法，用于研究 MS 对 PD 临床益处的潜在细胞生物学机制。
Glial cell-derived neurotrophic factor (GDNF) is a small protein potently promoting the survival of dopaminergic and motor neurons. GDNF can be secreted from different types of cells including the dopaminergic neural cell line, N27. N27 cells, a rat dopaminergic neural cell line, is regarded as a suitablein vitromodel for Parkinson's disease (PD) research. For PD treatment, transcranial magnetic stimulation (TMS), a noninvasive therapeutic method, showed beneficial clinical effects, but the mechanism for its benefit is not understood. Because GDNF is a potent neurotrophic factor, it is of great value to evaluate if GDNF secretion from N27 cells can be affected by magnetic stimulation (MS). However, the current methods for detecting GDNF are time-consuming and expensive. In this paper we outline the detection of GDNF secretion from N27 cells by ultrasensitive nanopore thin film sensors (nanosensor)for the first time. As low as 2 pg/mL GDNF can be readily detected by the nanosensor. Furthermore, we show that MS can promote GDNF secretion from N27 cells. Specifically, the GDNF concentration in N27 cell-conditioned media under MS treatment shows statistically significant increase up to 2-fold after 5 daysin vitroin comparison with the control. This nanosensor along with thein vitroPD model N27 cells provides a low-cost, easy-to-use, sensitive approach for studying potential cell biological mechanisms of the clinical benefits of MS on PD.