miR319, miR390, and miR393 Are Involved in Aluminum Response in Flax (Linum usitatissimum L.).
miR319, miR390, and miR393 Are Involved in Aluminum Response in Flax (Linum usitatissimum L.).
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DOI：
10.1155/2017/4975146
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发表时间：
2017
影响因子：
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通讯作者：
Melnikova NV
中科院分区：
文献类型：
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作者：
Dmitriev AA;Kudryavtseva AV;Bolsheva NL;Zyablitsin AV;Rozhmina TA;Kishlyan NV;Krasnov GS;Speranskaya AS;Krinitsina AA;Sadritdinova AF;Snezhkina AV;Fedorova MS;Yurkevich OY;Muravenko OV;Belenikin MS;Melnikova NV
酸性土壤限制了世界范围内的农业生产。在酸性土壤中，铝的毒性是造成作物损失的主要原因。在本工作中，我们研究了亚麻(Linum usitatisimum L.)铝胁迫下的植物。对Al抗性(TMP1919和G1071/4_k)和敏感(Lira和G1071/4_o)的亚麻幼苗分别在AlCl3溶液中处理4小时和24小时。利用Illumina平台构建了12个小RNA文库，并进行了测序。总共鉴定了来自18个保守家族的97个microRNAs。MiR319、miR390和miR393揭示了与铝处理亚麻植株相关的表达变化。此外，miR390和miR393在对铝的敏感和抗性上有明显的变化。定量聚合酶链式反应分析证实miR319和miR390的表达水平发生了变化。在亚麻中，miR319的潜在靶点是TCPs、miR390-TAS3和GRF5以及miR393-AFB2编码转录本。TCPs、TAS3、GRF5和AFB2参与植物生长发育的调控。首次发现miR319、miR390和miR393参与了亚麻对铝胁迫的响应。我们推测，这些microRNAs通过调节亚麻植株的生长过程，在Al反应中发挥重要作用。
Acid soils limit agricultural production worldwide. Major reason of crop losses in acid soils is the toxicity of aluminum (Al). In the present work, we investigated expression alterations of microRNAs in flax (Linum usitatissimum L.) plants under Al stress. Flax seedlings of resistant (TMP1919 and G1071/4_k) and sensitive (Lira and G1071/4_o) to Al cultivars and lines were exposed to AlCl3 solution for 4 and 24 hours. Twelve small RNA libraries were constructed and sequenced using Illumina platform. In total, 97 microRNAs from 18 conserved families were identified. miR319, miR390, and miR393 revealed expression alterations associated with Al treatment of flax plants. Moreover, for miR390 and miR393, the alterations were distinct in sensitive and resistant to Al genotypes. Expression level changes of miR319 and miR390 were confirmed using qPCR analysis. In flax, potential targets of miR319 are TCPs, miR390–TAS3 and GRF5, and miR393–AFB2-coding transcripts. TCPs, TAS3, GRF5, and AFB2 participate in regulation of plant growth and development. The involvement of miR319, miR390, and miR393 in response to Al stress in flax was shown here for the first time. We speculate that these microRNAs play an important role in Al response via regulation of growth processes in flax plants.