Non-destructive measurement of soil respiration in a grassland ecosystem using the multiple-microchambers method
Non-destructive measurement of soil respiration in a grassland ecosystem using the multiple-microchambers method
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DOI：
10.1007/s11284-018-1562-8
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发表时间：
2018-01
影响因子：
2
通讯作者：
Nobuhiko Suminokura;Mayuko Suzuki;K. Tanami;M. Tomotsune;S. Yoshitake;H. Koizumi
中科院分区：
文献类型：
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作者：
Nobuhiko Suminokura;Mayuko Suzuki;K. Tanami;M. Tomotsune;S. Yoshitake;H. Koizumi
摘要植物修剪箱法是草地生态系统土壤呼吸测定的常用方法。然而，植物修剪可能会导致高估SR通过改变环境因素和伤害植物。为了解决这些问题，我们开发了一种新的非破坏性的方法，使用多个微室（3厘米直径，8厘米高），这使得测量SR没有植物修剪安装到草之间的间隙室。在不同流速下，将新方法与传统方法进行了比较，以评估SR测量的准确性。新方法高估了SR速率;然而，对于每个流速，高估与传统方法的比率是恒定的。这些比率符合对数曲线，表明使用对数方程的新方法测量的SR率的校正潜力。新方法得到的校正SR率与传统方法得到的SR率相当。这表明，准确测量的SR在草地生态系统中使用多微室方法是可能的。在夏季对非破坏性方法和现场破坏性方法进行了比较，发现破坏性方法平均高估了草地生态系统的SR率约276%。有两个可能的原因，这种高估：第一，修剪处理可能会改变环境条件，如土壤温度和土壤水分含量，第二，它可能会直接增加植物呼吸。
The chamber method with plant clipping has been widely used for measuring soil respiration (SR) in grassland ecosystems. However, plant clipping may cause overestimation of SR by changing the environmental factors and injuring the plants. To solve these problems, we developed a new non-destructive method using multiple-microchambers (3 cm diameter, 8 cm height), which enables measurement of SR without plant clipping by installing chambers into gaps among the grasses. The new method was compared with the conventional method at various flow rates in vitro to assess the accuracy of SR measurement. The new method overestimated the SR rate; however, the ratio of overestimation to the conventional method was constant for each flow rate. These ratios fitted the logarithmic curve, indicating the potential for correction of the SR rate measured by the new method using the logarithmic equation. The corrected SR rate obtained by the new method was equal to the rate by the conventional method. This suggests that accurate measurement of SR in grassland ecosystems is possible using the multiple-microchambers method. We then compared the non-destructive method and the destructive method in situ on summer season and found that the destructive method overestimated SR rate in the grassland ecosystem by about 276% on average. There were two possible reasons for this overestimation; first, the clipping treatment may change environmental conditions such as soil temperature and soil water content, and second, it may directly increase plant respiration.