A detached seedling leaf technique to study resistance to Mycosphaerella graminicola (anamorph Septoria tritici) in wheat
A detached seedling leaf technique to study resistance to Mycosphaerella graminicola (anamorph Septoria tritici) in wheat
复制标题
DOI：
10.1046/j.1365-3059.2001.00570.x
复制
发表时间：
2001-06-01
期刊：
影响因子：
2.7
通讯作者：
Brown, JKM
中科院分区：
文献类型：
--
作者：
Arraiano, LS;Brading, PA;Brown, JKM
采用离体苗叶技术筛选小麦对壳针孢斑点病的抗性，并检测品种与菌株间的特异性互作。然后将分离的初生叶置于透明塑料盒中，使得它们的切割端夹在含有苯并咪唑的琼脂层之间，在叶的中间下方具有间隙。平均发病水平受光照和温度的影响，也受苯并咪唑浓度的影响，因此浓度越高，发病率越低。次生叶比幼苗初生叶更感病。然而，没有这些因素影响品种之间的疾病或品种隔离相互作用的排名。Kavkaz-K4500 1.6.a.4、Synthetic 6x和Macha对不同的菌株表现出特异的感病性和抗性。离体叶片技术可以是一个有用的补充，田间试验和替代全苗测定在评估品种的抗性和调查的宿主-病原体相互作用的遗传。
A detached seedling leaf technique was developed to screen for resistance to septoria tritici blotch of wheat and to detect specific interactions between cultivars and isolates, Wheat seedlings were inoculated with spore suspensions of Mycosphaerella graminicola. Detached primary leaves were then placed in a clear plastic box such that their cut ends were sandwiched between layers of agar containing benzimidazole, with a gap below the middle of the leaves. Mean levels of disease were affected by light and temperature, and also by the concentration of benzimidazole, such that higher concentrations resulted in less disease. Second leaves were more susceptible than seedling primary leaves. However, none of these factors affected ranking of disease among cultivars or cultivar-by-isolate interactions. Kavkaz-K4500 1.6.a.4, Synthetic 6x and Triticum macha showed specific susceptibility and resistance to different isolates. The detached leaf technique could be a useful complement to field trials and an alternative to whole seedling assays in assessing cultivar resistance and investigating the genetics of the host-pathogen interaction.