Arabidopsis thaliana MLO genes are expressed in discrete domains during reproductive development
Arabidopsis thaliana MLO genes are expressed in discrete domains during reproductive development
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DOI：
10.1007/s00497-017-0313-2
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发表时间：
2017-12-01
影响因子：
3.4
通讯作者：
Kessler, Sharon A.
中科院分区：
文献类型：
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作者：
Davis, Thomas C.;Jones, Daniel S.;Kessler, Sharon A.
抗白粉病蛋白-O（MLO）家族由15个成员组成，在不同的细胞间通讯过程中发挥作用，如白粉病易感性，根thigmomorphogenesis，和花粉管接收。NORTIA（NTA，AtML 0 7）基因在雌配子体的助细胞中表达，在那里它与花粉管进行细胞间通讯。先前发表的启动子：：GUS和启动子：：基因-GUS构建体表达模式之间的差异使我们探索NTA表达的调控。在这里，我们发现通过NTA（pro）：：gNTA-GUS截短，NTA基因内的序列负调控其在气孔和心皮壁中的表达。这导致了一种假设，即其他MLO家族成员也可能在基因内具有额外的调控序列。对每个家族成员的MLOpro：：gMLO-GUS构建体进行检查，特别关注花，以确定其他MLO是否可以在生殖细胞-细胞通讯中发挥作用。值得注意的是，几种MLO在花粉、柱头、授粉花柱以及助细胞和中央细胞中表达。这些发现表明，除了NTA以外，其他MLO也可能在生殖中发挥作用。以往对MLO家族的研究表明，在白粉病感染和根的thigmomorphogenesis中，遗传相关的MLO具有冗余的功能;然而，MLO在生殖组织中的表达并不严格遵循系统发育关系，这表明来自不同进化起源的MLO可能被招募用于有性生殖功能。
The MILDEW RESISTANCE LOCUS-O (MLO) protein family, comprised of 15 members, plays roles in diverse cell-cell communication processes such as powdery mildew susceptibility, root thigmomorphogenesis, and pollen tube reception. The NORTIA (NTA, AtMLO7) gene is expressed in the synergid cells of the female gametophyte where it functions in intercellular communication with the pollen tube. Discrepancies between previously published promoter::GUS and promoter::gene-GUS constructs expression patterns led us to explore the regulation of NTA expression. Here we found via NTA(pro)::gNTA-GUS truncations that sequences within the NTA gene negatively regulate its expression in the stomata and carpel walls. This led to the hypothesis that other MLO family members may also have additional regulatory sequences within the gene. MLOpro::gMLO-GUS constructs were examined for each family member focusing specifically on flowers in order to determine whether other MLOs could play a role in reproductive cell-cell communication. Notably, several MLOs were expressed in the pollen, in the stigma, in the pollinated style, and in the synergids and central cell. These findings indicate that other MLOs in addition to NTA could play a role in reproduction. Previous studies on the MLO family showed that phylogenetically related MLOs had redundant functions in powdery mildew infection and root thigmomorphogenesis; however, MLO expression in reproductive tissues did not strictly follow phylogenetic relationships, indicating that MLOs from different evolutionary origins may have been recruited for function in sexual reproduction.