Genome-wide sequencing of longan (Dimocarpus longan Lour.) provides insights into molecular basis of its polyphenol-rich characteristics.
Genome-wide sequencing of longan (Dimocarpus longan Lour.) provides insights into molecular basis of its polyphenol-rich characteristics.
复制标题
龙眼（Dimocarpus longan Lour.）的全基因组测序提供了对其富含多酚特征的分子基础的见解
DOI：
10.1093/gigascience/gix023
复制
发表时间：
2017-05-01
期刊：
影响因子：
9.2
通讯作者：
Lai Z
中科院分区：
文献类型：
--
作者：
Lin Y;Min J;Lai R;Wu Z;Chen Y;Yu L;Cheng C;Jin Y;Tian Q;Liu Q;Liu W;Zhang C;Lin L;Zhang D;Thu M;Zhang Z;Liu S;Zhong C;Fang X;Wang J;Yang H;Varshney RK;Yin Y;Lai Z
摘要龙眼（Dimocarpus longan Lour.），无患子科一种重要的亚热带水果，在10多个国家种植。龙眼是一种可食用的干果，也是一种富含多酚的传统药物来源。树的大小，轮流结果，和丛枝病仍然是严重的问题。为了深入了解龙眼性状的基因组基础，组装了基因组序列草图。中国龙眼品种“红盒子”基因组草图（约471.88Mb）包含31007个基因和261.88Mb的重复序列。在基因组中没有检测到最近的全基因组范围的重复事件。对13个栽培D.龙眼种质资源的遗传多样性程度较高。比较转录组研究与全基因组分析相结合，揭示了多酚丰富和病原体抗性的特点。参与次生代谢的基因，特别是那些显着扩大（DHS，SDH，F3 β-羟甲基化酶，ANR，和UFGT）和收缩（PAL，CHS，和F3 β-5 β-羟甲基化酶）基因家族的组织特异性表达，可能是重要的贡献者，在龙眼果实中观察到的多酚化合物的高积累水平。大量的基因编码的核苷酸结合位点亮氨酸丰富的重复序列（NBS-LRR）和亮氨酸丰富的重复受体样激酶蛋白，以及最近的扩展和收缩的NBS-LRR家族，建议在这果树抗昆虫，真菌和细菌的基因组基础。这些数据为深入了解龙眼基因组的进化和多样性提供了帮助。比较基因组和转录组分析提供了有关龙眼特有性状的信息，特别是涉及其富含多酚和病原体抗性特征的基因。
Abstract Longan (Dimocarpus longan Lour.), an important subtropical fruit in the family Sapindaceae, is grown in more than 10 countries. Longan is an edible drupe fruit and a source of traditional medicine with polyphenol-rich traits. Tree size, alternate bearing, and witches' broom disease still pose serious problems. To gain insights into the genomic basis of longan traits, a draft genome sequence was assembled. The draft genome (about 471.88 Mb) of a Chinese longan cultivar, “Honghezi,” was estimated to contain 31 007 genes and 261.88 Mb of repetitive sequences. No recent whole-genome-wide duplication event was detected in the genome. Whole-genome resequencing and analysis of 13 cultivated D. longan accessions revealed the extent of genetic diversity. Comparative transcriptome studies combined with genome-wide analysis revealed polyphenol-rich and pathogen resistance characteristics. Genes involved in secondary metabolism, especially those from significantly expanded (DHS, SDH, F3΄H, ANR, and UFGT) and contracted (PAL, CHS, and F3΄5΄H) gene families with tissue-specific expression, may be important contributors to the high accumulation levels of polyphenolic compounds observed in longan fruit. The high number of genes encoding nucleotide-binding site leucine-rich repeat (NBS-LRR) and leucine-rich repeat receptor-like kinase proteins, as well as the recent expansion and contraction of the NBS-LRR family, suggested a genomic basis for resistance to insects, fungus, and bacteria in this fruit tree. These data provide insights into the evolution and diversity of the longan genome. The comparative genomic and transcriptome analyses provided information about longan-specific traits, particularly genes involved in its polyphenol-rich and pathogen resistance characteristics.