JACKDAW controls epidermal patterning in the Arabidopsis root meristem through a non-cell-autonomous mechanism
JACKDAW controls epidermal patterning in the Arabidopsis root meristem through a non-cell-autonomous mechanism
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DOI：
10.1242/dev.048777
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发表时间：
2010-05-01
期刊：
影响因子：
4.6
通讯作者：
Blilou, Ikram
中科院分区：
文献类型：
--
作者：
Hassan, Hala;Scheres, Ben;Blilou, Ikram
在拟南芥中，毛发和非毛发表皮细胞类型的规格是位置依赖性的，因为毛细胞出现在下面的皮质细胞层的裂缝上。表皮图案是由转录调节因子网络决定的，该网络对来自底层组织的未知信号做出反应。此前，我们发现 JACKDAW (JKD) 是一种锌指蛋白，定位于静止中心和基底组织，并通过限制短根运动来调节组织边界和不对称细胞分裂。在这里，我们提供证据表明截拳道控制调节表皮细胞类型模式的位置依赖性信号。 JKD 是表皮细胞命运调节因子 GLABRA2、CAPRICE 和 WEREWOLF 的适当模式表达所必需的。遗传相互作用研究表明，JKD 在胚胎发生后以 SCRAMBLED (SCM) 依赖性方式在表皮图案网络的上游运作，但在胚胎发生中的作用独立于 SCM。组织特异性诱导实验表明，JKD 从底层皮层细胞层起非细胞自主作用，以指定表皮细胞的命运。我们的研究结果与截拳道在每个皮层细胞中诱导信号的模型一致，由于位于裂口上方的细胞表面接触较大，因此毛细胞位置的信号更为丰富。
In Arabidopsis, specification of the hair and non-hair epidermal cell types is position dependent, in that hair cells arise over clefts in the underlying cortical cell layer. Epidermal patterning is determined by a network of transcriptional regulators that respond to an as yet unknown cue from underlying tissues. Previously, we showed that JACKDAW (JKD), a zinc finger protein, localizes in the quiescent centre and the ground tissue, and regulates tissue boundaries and asymmetric cell division by delimiting SHORT-ROOT movement. Here, we provide evidence that JKD controls position-dependent signals that regulate epidermal-cell-type patterning. JKD is required for appropriately patterned expression of the epidermal cell fate regulators GLABRA2, CAPRICE and WEREWOLF. Genetic interaction studies indicate that JKD operates upstream of the epidermal patterning network in a SCRAMBLED (SCM)-dependent fashion after embryogenesis, but acts independent of SCM in embryogenesis. Tissue-specific induction experiments indicate non-cell-autonomous action of JKD from the underlying cortex cell layer to specify epidermal cell fate. Our findings are consistent with a model where JKD induces a signal in every cortex cell that is more abundant in the hair cell position owing to the larger surface contact of cells located over a cleft.