Hydrophobic silver nanoparticles trapped in lipid bilayers: Size distribution, bilayer phase behavior, and optical properties.
Hydrophobic silver nanoparticles trapped in lipid bilayers: Size distribution, bilayer phase behavior, and optical properties.
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DOI：
10.1186/1477-3155-6-13
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发表时间：
2008-11-12
影响因子：
10.2
通讯作者：
Bothun GD
中科院分区：
文献类型：
--
作者：
Bothun GD
纳米颗粒的基于脂质的分散体提供了设计治疗剂的生物启发途径和降低纳米颗粒毒性的手段。目前对纳米颗粒的存在如何影响脂质囊泡稳定性和双层相行为知之甚少。在这项工作中，形成的水性脂质/纳米粒子组件（LNA）的疏水性银-癸烷醇颗粒（5.7 ± 1.8 nm）嵌入1，2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）双层被证明作为DPPC/银纳米粒子（AgNP）的比例的函数。纳米粒子负载的大小分布，双层相行为，和双层流动性的影响进行了测定。同时，双层掺入的AgNP的光学性质的影响也检查。分散体在50°C下是稳定的，其中双层以液晶状态存在，但在25°C下相分离，其中双层处于凝胶状态，这与低于脂质熔融温度的囊泡聚集一致。通过差示扫描量热法和荧光各向异性证实了双层嵌入的纳米颗粒的形成，其中增加纳米颗粒浓度抑制了脂质预转变温度，降低了熔融温度，并破坏了凝胶相双层。包埋纳米粒子的特征表面等离子体共振（SPR）波长是独立的双层相，但是，SPR吸光度依赖于囊泡聚集。这些结果表明，脂质双层可以扭曲，以适应大的疏水纳米粒子，相对于双层的厚度，并可能提供洞察纳米粒子/生物膜的相互作用和多功能脂质体载体的设计。
Lipid-based dispersion of nanoparticles provides a biologically inspired route to designing therapeutic agents and a means of reducing nanoparticle toxicity. Little is currently known on how the presence of nanoparticles influences lipid vesicle stability and bilayer phase behavior. In this work, the formation of aqueous lipid/nanoparticle assemblies (LNAs) consisting of hydrophobic silver-decanethiol particles (5.7 ± 1.8 nm) embedded within 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers is demonstrated as a function of the DPPC/Ag nanoparticle (AgNP) ratio. The effect of nanoparticle loading on the size distribution, bilayer phase behavior, and bilayer fluidity is determined. Concomitantly, the effect of bilayer incorporation on the optical properties of the AgNPs is also examined. The dispersions were stable at 50°C where the bilayers existed in a liquid crystalline state, but phase separated at 25°C where the bilayers were in a gel state, consistent with vesicle aggregation below the lipid melting temperature. Formation of bilayer-embedded nanoparticles was confirmed by differential scanning calorimetry and fluorescence anisotropy, where increasing nanoparticle concentration suppressed the lipid pretransition temperature, reduced the melting temperature, and disrupted gel phase bilayers. The characteristic surface plasmon resonance (SPR) wavelength of the embedded nanoparticles was independent of the bilayer phase; however, the SPR absorbance was dependent on vesicle aggregation. These results suggest that lipid bilayers can distort to accommodate large hydrophobic nanoparticles, relative to the thickness of the bilayer, and may provide insight into nanoparticle/biomembrane interactions and the design of multifunctional liposomal carriers.