Effect of exposure to injection drugs or alcohol on antigen-specific immune responses in HIV and hepatitis C virus coinfection.
Effect of exposure to injection drugs or alcohol on antigen-specific immune responses in HIV and hepatitis C virus coinfection.
复制标题
接触注射药物或酒精对艾滋病毒和丙型肝炎病毒合并感染中抗原特异性免疫反应的影响。
DOI：
10.1086/511990
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Koziel,MargaretJames
中科院分区：
文献类型：
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作者：
Graham,CamillaS;Wells,Annalee;Edwards,ErikaM;Herren,Timothy;Tumilty,Sheila;Stuver,SherriO;Samet,JeffreyH;Nunes,David;HorsburghJr,CRobert;Koziel,MargaretJames
背景：持续的药物使用是丙型肝炎病毒（HCV）免疫学研究的潜在混杂因素，但关于注射吸毒（IDU）或酒精对HCV特异性免疫应答影响的文献很少。我们要确定是否IDU或酒精影响免疫反应，HCV感染和人类免疫缺陷病毒（HIV）/HCV coinfected subjects.Methods.Eight-four与HIV/HCV和57与HCV被归类为注射吸毒者，饮酒者，或非用户的问卷调查结果的基础上。用酶联免疫吸附斑点试验研究了干扰素（IFN）-γ、白细胞介素（IL）-10和肿瘤坏死因子（TNF）-α对HCV核心蛋白、NS 3和NS 5以及回忆抗原的免疫应答。然而，HIV/HCV注射吸毒者的HCV特异性IFN-γ和IL-10反应与HCV注射吸毒者相似，且显著高于非HIV/HCV注射吸毒者。相反，饮酒的受试者有类似的免疫反应，那些禁欲的，受试者与HIV/HCV和受试者与HCV.Conclusions.Studies研究IFN-γ或IL-10免疫反应在HIV/HCV共感染或HCV感染的人需要考虑目前的IDU。酒精，在这个群体中消费的水平，似乎对抗原特异性免疫反应没有太大的影响。
Background.Ongoing substance use is a potential confounder for immunological studies on hepatitis C virus (HCV), but there is little in the literature regarding the effects of injection drug use (IDU) or alcohol on HCVspecific immune responses. We wanted to determine whether IDU or alcohol affected immune responses in HCVinfected and human immunodeficiency virus (HIV)/HCV coinfected subjects.Methods.Eight-four subjects with HIV/HCV and 57 with HCV were classified as either injection drug users, drinkers, or nonusers based on questionnaire results. Immune responses were studied with enzyme-linked immunosorbent spot assay for interferon (IFN)-γ, interleukin (IL)-10, and tumor necrosis factor (TNF)-α against HCV proteins Core, NS3, and NS5 and recall antigens.Results.Subjects with HIV/HCV, in aggregate, had significantly lower HCV-specific IFN-γ and TNF-α responses than subjects with HCV. However, HIV/HCV injection drug users had HCV-specific IFN-γ and IL-10 responses that were similar to those of HCV injection drug users and were significantly higher than in nonusers with HIV/HCV. Conversely, subjects who drank alcohol had similar immune responses to those who were abstinent, among both subjects with HIV/HCV and subjects with HCV.Conclusions.Studies that examine IFN-γ or IL-10 immune responses in HIV/HCV-coinfected orHCV-infected persons need to consider current IDU. Alcohol, at levels consumed in this cohort, does not appear to have as much of an effect on antigen-specific immune responses.