Periostin and bone marrow fibrosis
Periostin and bone marrow fibrosis
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DOI：
10.1007/s12185-008-0095-2
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发表时间：
2008-07-01
影响因子：
2.1
通讯作者：
Okamura, Takashi
中科院分区：
文献类型：
--
作者：
Oku, Eijiro;Kanaji, Taisuke;Okamura, Takashi
骨膜蛋白是一种分泌性蛋白质，与昆虫轴突导向蛋白fasciclin 1具有结构同源性。骨膜蛋白主要表达于受到恒定机械应力的富含胶原的纤维结缔组织中。我们以前已经证明骨膜蛋白是支气管哮喘上皮下纤维化的一种新成分。在这里，我们研究了骨膜蛋白和骨髓（BM）纤维化之间的关系。骨膜蛋白在BM纤维化标本的基质和基质细胞中表达，并且其表达水平在很大程度上与纤维化的分级密切相关，如通过银染色所估计的。然而，在本研究中，我们发现血浆骨膜蛋白水平与BM纤维化程度之间没有关系。我们还证明了骨膜蛋白由人BM hTERT基质细胞分泌，并且其分泌被TGF-β（巨核细胞和/或单核细胞的克隆增殖产生的细胞因子）增强。这些结果表明，骨膜蛋白是BM纤维化的组分，并且它可能在疾病进展中起作用。
Periostin is a secreted protein that shares structural homology with the insect axon guidance protein fasciclin 1. Periostin is expressed predominantly in collagen-rich fibrous connective tissues that are subjected to constant mechanical stresses. We have shown previously that periostin is a novel component of subepithelial fibrosis in bronchial asthma. Here, we investigated the relationship between periostin and bone marrow (BM) fibrosis. Periostin was expressed in the stroma and stromal cells of BM fibrosis specimens and to a great extent its expression levels correlated closely to the grade of fibrosis, as estimated by silver staining. However, in the present study, we found no relationship between plasma periostin levels and the extent of BM fibrosis. We also demonstrated that periostin is secreted by human BM hTERT stromal cells and that its secretion is enhanced by TGF-beta, a cytokine produced by clonal proliferation of megakaryocytes and/or monocytes. These results indicate that periostin is a component of BM fibrosis and that it may play a role in the disease progression.