Mfsd2a and Glut1 Brain Nutrient Transporters Expression Increase with 32-Week Low and High Lard Compared with Fish-Oil Dietary Treatment in C57Bl/6 Mice.
Mfsd2a and Glut1 Brain Nutrient Transporters Expression Increase with 32-Week Low and High Lard Compared with Fish-Oil Dietary Treatment in C57Bl/6 Mice.
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DOI：
10.1093/cdn/nzy065
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发表时间：
2018-10
影响因子：
4.8
通讯作者：
Witt KA
中科院分区：
文献类型：
--
作者：
Sandoval KE;Wooten JS;Harris MP;Schaller ML;Umbaugh DS;Witt KA
饮食介导的关键脑营养转运蛋白的改变，主要促进剂超家族结构域包含2a（Mfsd 2a）和葡萄糖转运蛋白1（Glut 1），在脑健康和疾病中具有广泛的意义。这项研究的目的是检查长期低脂肪和高脂肪饮食与猪油或鱼油对关键脑营养转运蛋白Mfsd 2a和Glut 1的影响。八周龄雄性C57 BL/6小鼠喂食以下4种饮食中的1种，持续32周：10%的大卡来自猪油，10%的大卡来自鱼油，41%的大卡来自猪油，或41%的大卡来自鱼油。体重和血液化学描述了饮食的影响。皮质和皮质下Mfsd 2a和Glut 1 mRNA和蛋白表达进行了评估，其他支持性营养敏感的目标也评估了mRNA表达的变化。鱼油饮食增加皮质Mfsd 2a mRNA的表达与猪油饮食相比。皮质下Mfsd 2a mRNA表达随着饮食中脂肪百分比的增加而降低。脂肪的类型和百分比与皮质和皮质下Mfsd 2a和皮质Glut 1蛋白表达之间存在相互作用。在猪油饮食组中，皮质和皮质下Mfsd 2a和皮质Glut 1的蛋白表达随着脂肪百分比的增加而显著增加。随着鱼油饮食组中脂肪百分比的增加，皮质和皮质下Mfsd 2a和皮质Glut 1的蛋白表达没有变化。当比较鱼油组与10%猪油，皮质Mfsd 2a蛋白表达显着较高的10%和41%的鱼油组，而皮质Glut 1蛋白表达显着较高的只有10%的鱼油组。皮质过氧化物酶体增殖物激活受体γ mRNA表达与Mfsd 2a蛋白表达呈正相关。与慢性饮食治疗相对应，脂肪类型和脂肪百分比之间存在相互作用，分别与关键营养转运蛋白Mfsd 2a和Glut 1的脑表达变化有关。
Diet-mediated alterations of critical brain nutrient transporters, major facilitator super family domain-containing 2a (Mfsd2a) and glucose transporter 1 (Glut1), have wide reaching implications in brain health and disease. The aim of the study was to examine the impact of long-term low- and high-fat diets with lard or fish oil on critical brain nutrient transporters, Mfsd2a and Glut1. Eight-week-old male C57BL/6 mice were fed 1 of the following 4 diets for 32 wk: 10% of kcal from lard, 10% of kcal from fish oil, 41% of kcal from lard, or 41% of kcal from fish oil. Body weight and blood chemistries delineated dietary effects. Cortical and subcortical Mfsd2a and Glut1 mRNA and protein expression were evaluated, with other supportive nutrient-sensitive targets also assessed for mRNA expression changes. Fish-oil diets increased cortical Mfsd2a mRNA expression compared with lard diets. Subcortical Mfsd2a mRNA expression decreased as the percentage of fat in the diet increased. There was an interaction between the type and percentage of fat with cortical and subcortical Mfsd2a and cortical Glut1 protein expression. In the lard diet groups, protein expression of cortical and subcortical Mfsd2a and cortical Glut1 significantly increased as fat percentage increased. As the fat percentage increased in the fish-oil diet groups, protein expression of cortical and subcortical Mfsd2a and cortical Glut1 did not change. When comparing the fish-oil groups with 10% lard, cortical Mfsd2a protein expression was significantly higher in the 10% and 41% fish-oil groups, whereas cortical Glut1 protein expression was significantly higher in only the 10% fish-oil group. A positive correlation between cortical peroxisome proliferator–activated receptor γ mRNA expression and Mfsd2a protein expression was shown. Corresponding to chronic dietary treatment, an interaction between the type of fat and the percentage of fat exists respective to changes in brain expression of the key nutrient transporters Mfsd2a and Glut1.