Effect of Ono-EI-600 elastase inhibitor on high-tidal-volume-induced lung injury in rats.
Effect of Ono-EI-600 elastase inhibitor on high-tidal-volume-induced lung injury in rats.
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DOI：
10.1007/s00540-005-0369-2
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Nishimura, Masaji
中科院分区：
文献类型：
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作者：
Fujita, Yasuki;Fujino, Yuji;Nishimura, Masaji
我们在大鼠模型中测试了Ono-EI-600（一种抑制细胞因子释放的弹性蛋白酶抑制剂）对呼吸机诱导的肺损伤的影响。Wistar大鼠术后（8-11周龄）随机分为4组：高潮气量（V（T））组H组：n = 10，吸气峰压（PIP）30 cmH 2 O，持续240 min;（HD组：n = 10）接受与H组相同的通气设置，并在方案期间静脉输注10 mg x kg（-1）x h（-1）Ono-EI-600;较低V（T）组H组支气管肺泡灌洗液（BALF）中细胞因子IL-6和CINC-1水平显著高于C组和L组（P < 0.05）。然而，对于H组和HD组，动脉血气数据、BALF中细胞因子水平和组织学损伤评分无差异。我们的实验没有提供弹性蛋白酶抑制剂Ono-EI-600对高V（T）通气诱导的肺损伤有保护作用的证据。
We tested the effect of Ono-EI-600, an elastase inhibitor that suppresses cytokine release, on ventilator-induced lung injury in a rat model. After Wistar rats (aged 8-11 weeks) were anesthetized and tracheostomized, they were randomly assigned to four groups: high tidal volume (V(T)) group (H group: n = 10) receiving peak inspiratory pressure (PIP) 30 cmH(2)O for 240 min; high V(T) with drug group (HD group: n = 10) receiving the same ventilation settings as H group and also intravenous infusion 10 mg x kg(-1) x h(-1) of Ono-EI-600 during the protocol; the lower V(T) group (L group: n = 5) receiving PIP 10 cmH(2)O for 240 min; and control group (C group: n = 5) receiving the same ventilation as L group for 30 min. The cytokine levels (IL-6 and CINC-1) in the bronchoalveolar lavage fluid (BALF) of the H group were significantly higher than those of the C and L groups (P < 0.05). However, for the H and HD groups, no differences were found in arterial blood gas data, cytokine levels in BALF, and histological injury scores. Our experiment provided no evidence that elastase inhibitor Ono-EI-600 protects against lung injury induced by high V(T) ventilation.