Prevalence and characterization of Salmonella infantis isolates originating from different points of the broiler chicken-human food chain in Hungary
Prevalence and characterization of Salmonella infantis isolates originating from different points of the broiler chicken-human food chain in Hungary
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DOI：
10.1016/j.ijfoodmicro.2008.07.005
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发表时间：
2008-09-30
影响因子：
5.4
通讯作者：
Kiss, I.
中科院分区：
文献类型：
--
作者：
Nogrady, N.;Kardos, G.;Kiss, I.
在10个月的研究期间，三个农场（A。B和C），屠宰家禽的屠宰场，以及来自这些屠宰场的屠体和零售生肉产品。在肉鸡养殖场A中，对5个连续的鸡群进行采样，在B和C养殖场中，对1个鸡群进行采样。在引入之前采集了环境样本。使用标准方法定期收集环境、饮用水、饲料和粪便样品。在处理鸡群之前和期间，在屠宰场采集了环境和尸体样本。还检查了屠体、零售肉类以及农场和屠宰场工人粪便样本的沙门氏菌污染，以及同期和地区登记的人类疾病。分离、血清、噬菌体和抗生素耐药性分型。进行菌株的1类整合子和质粒谱分析：通过PFGE评估它们的遗传关系。A场的鸡舍和5个鸡群的粪便样品均为沙门氏菌阴性，但S。从20-100%的屠宰场尸体样品中分离出了沙门氏菌。零售生肉样品为0-100% S。是的。养殖场B的环境样品均为沙门氏菌阴性，但被检鸡群受到沙门氏菌污染。从43%的粪便样本中鉴定出了沙门氏菌。该血清型在100%的屠体和零售生肉样本中被鉴定。从1日龄雏鸡到达肉鸡舍C、S之前采集的环境样品中，他是有文化的。S.粪便样本中沙门氏菌的感染率为35%，所有的胴体和零售生肉样本均为沙门氏菌。已经被污染了。共164个S。分离出145株Escherichia coli菌株，并对其进行了进一步鉴定。绝大多数菌株（142/145）属于噬菌体217和213型。除一个外，所有的特征在于萘啶酸-链霉素-磺酰胺-四环素抗性，具有885 bp的1类整合子和大小> 168 kb的大质粒。菌株间遗传相似性>= 88.7%。结果表明，同一株多重耐药的S。从被检查的肉鸡养殖场传播的克隆污染了屠宰和零售肉，并出现在被检查地区的人类疾病中，早期被检测为全国肉鸡和人类种群的主要克隆特征。(C)2008 Elsevier B. V.保留所有权利。
During the 10-month study period Salmonella contamination of broiler houses and the flocks reared in three farms (A. B and C), the slaughter houses where the flocks were slaughtered, as well as the carcass and retail raw meat products originating from them was investigated. In the broiler farm A five consecutive flocks, in the B and C farms one flock was sampled. Environmental samples were taken prior to the introductions. Environmental, drinking water, feed and faecal samples were collected regularly using standard methods. Before and during processing of the flocks, environmental and carcass samples were taken at the abattoirs. Salmonella contamination of the carcass, retail meat, as well as stool samples of farm and abattoir workers and from human illnesses registered in the same period and region were also examined. Isolation, sero-, phage- and antibiotic resistance typing. class 1 integron and plasmid profiling of the strains were performed: their genetic relationship was assessed by PFGE. Although the broiler house and the faecal samples of the 5 flocks of the farm A were negative for Salmonella, S. infantis was isolated from 20-100% of the abattoir carcass samples. The retail raw meat samples were 0-100% S. infantis positive. The environmental samples of farm B were Salmonella negative, but the examined flock was contaminated: S. infantis was identified from 43% of the faecal samples. This serotype was identified in 100% of the carcass and retail raw meat samples. From environmental samples taken before the arrival of the 1-day-old chicks in the broiler house C, S. infantis was cultured. S. infantis prevalence in the faecal samples was 35% and all the carcass and retail raw meat samples were S. infantis contaminated. Altogether 164 S. infantis strains were isolated out of which 145 were further characterized. The vast majority (142/145) of the strains belonged to phage types 217 and 213. All but one were characterized by the nalidixic acid-streptomycin-sulphonamide-tetracycline resistances, had an 885 bp class 1 integron and a large plasmid of > 168 kb in size. The strains showed >= 88.7% genetic similarity. The results obtained shows that the same multi-drug resistant S. infantis clone was spread from the examined broiler farms contaminating the slaughter and the retail meat and appeared in the human illnesses of the examined region that was earlier detected as the dominant clone characteristic of the broiler and human population of the whole country. (C) 2008 Elsevier B.V. All rights reserved.