Effects of low-power laser irradiation on the threshold of electrically induced paroxysmal discharge in rabbit hippocampus CA1
Effects of low-power laser irradiation on the threshold of electrically induced paroxysmal discharge in rabbit hippocampus CA1
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DOI：
10.1007/s10103-009-0681-4
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发表时间：
2009
影响因子：
2.1
通讯作者：
S. Kogure;Satoshi Takahashi;N. Saito;Kazuya Kozuka;Yoshiki Matsuda
中科院分区：
文献类型：
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作者：
S. Kogure;Satoshi Takahashi;N. Saito;Kazuya Kozuka;Yoshiki Matsuda
在急性实验中，使用成年家兔，我们测量了阵发性放电阈值（PADT）引起的刺激前和后的低功率激光照射海马CA 1区的顶端树突层。将Nd：YVO 4激光照射（波长：532 nm）引入与刺激部位相同的区域。激光照射前平均PADT为247 ± 13 μA（n= 18），而50、75和100 mW激光照射5 min后，PADT分别为333 ± 40（n= 4）、353 ± 33（n= 4）和367 ± 27 μA（n= 6）。后两种增量与对照组相比有统计学意义（p< 0.05和p < 0.01）。在75和100 mW激光照射10 min后，PADT分别为340 ± 47（n= 9）和480 ± 60 μA（n= 11;p< 0.01）。用特定波长和平均功率的激光照射家兔海马CA 1区，可以抑制阵发性放电的产生。相关分析表明，PADT增量是基于光化学以及激光照射的光热效应。
In acute experiments using adult rabbits, we measured the paroxysmal discharge threshold (PADT) elicited by stimulation to the apical dendritic layer of the hippocampal CA1 region before and after low-power laser irradiation. Nd:YVO4laser irradiation (wavelength: 532 nm) was introduced into the same region as the stimulation site. The average PADT was 247 ± 13 μA (n= 18) before laser irradiation, while after 5-min laser irradiation with 50, 75, and 100 mW, PADT was 333 ± 40 (n= 4), 353 ± 33 (n= 4) and 367 ± 27 μA (n= 6), respectively. The latter two increments were statistically significant compared to the control (p< 0.05 andp< 0.01). After 10-min laser irradiation with 75 and 100 mW, PADT was 340 ± 47 (n= 9) and 480 ± 60 μA (n= 11;p< 0.01), respectively. Laser irradiation with a specific wavelength and average power offers the potential to suppress the generation of paroxysmal discharges in rabbit hippocampus CA1. Correlation analyses suggest that PADT increments are based on photochemical as well as photothermal effects of laser irradiation.