Thermotropic liquid crystals as substrates for imaging the reorganization of matrigel by human embryonic stem cells
Thermotropic liquid crystals as substrates for imaging the reorganization of matrigel by human embryonic stem cells
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DOI：
10.1002/adfm.200500768
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发表时间：
2006-03-20
影响因子：
19
通讯作者：
Abbott, NL
中科院分区：
文献类型：
--
作者：
Lockwood, NA;Mohr, JC;Abbott, NL
我们研究了人胚胎干细胞（hESCs）在热致液晶TL205界面上的培养，TL205表面装饰有细胞外基质Matrigel薄膜。在液晶/基质界面上播种的hESCs可以存活数周，细胞集落在这段时间内生长。细胞的分化水平与在基质涂层玻璃上观察到的细胞相当。偏振显微镜和荧光显微镜显示，液晶的取向顺序与矩阵的存在和组织是耦合的。这使得hESCs通过使用偏振光显微镜观察液晶外观的变化来直接成像矩阵的重组。因此，Matrigel和TL205之间的耦合为监测Matrigel薄膜随时间的重组提供了一个简单的工具。我们的结果为细胞培养和细胞-细胞外-基质相互作用的测量提供了新的方法。
We have investigated the culture of human embryonic stem cells (hESCs) on interfaces of the thermotropic liquid crystal, TL205, that are decorated with thin films of the extracellular matrix, Matrigel. hESCs seeded at the liquid-crystal/Matrigel interface survive for weeks, and cell colonies grow over this time. The cells show levels of differentiation comparable to that observed for cells on Matrigel-coated glass controls. Polarized and fluorescence microscopy reveal that the orientational order of the liquid crystal is coupled to the presence and organization of Matrigel. This enables straightforward imaging of the reorganization of Matrigel by the hESCs through changes in the appearance of the liquid crystal when observed using polarized light microscopy. The coupling between Matrigel and TL205 thus provides a simple tool for monitoring the reorganization of the Matrigel film over time. Our results suggest new approaches to the culture of cells and measurements of cell-extracellular-matrix interactions.