Population structure of Chinese southwest wheat germplasms resistant to stripe rust and powdery mildew using the DArT-seq technique
Population structure of Chinese southwest wheat germplasms resistant to stripe rust and powdery mildew using the DArT-seq technique
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利用 DArT-seq 技术研究中国西南小麦抗条锈病和白粉病种质的群体结构
DOI：
10.1590/0103-8478cr20160066
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Zhang, Liyi
中科院分区：
文献类型：
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作者：
Chen, Tianqing;Tantasawat, Piyada Alisha;Zhang, Liyi
了解现有小麦种质资源的遗传变异对小麦种质资源的收集、保护和利用具有重要意义。利用Pm21和Yr26两个抗性基因标记和DArT-seq技术，对138份中国西南小麦材料进行了基因分型研究。50%的品种（系）扩增到了Yr26基因（Gwm11）的特异等位基因，40.6%的品种（系）扩增到了Pm21基因（SCAR1265）的特异等位基因。通过DArT-seq分析，共获得30485个标记（6486个SNP和23999个DArT），其中DArT和SNP标记的平均多态性信息含量（PIC）分别为0.33和0.28。平均遗传相似系数（GS）为0.72。利用主坐标分析（PCoA）分别在6AS染色体和全基因组水平上鉴定出两组一致的小麦品种。I组由不同来源的非6VS/6AL易位系组成，II组由6VS/6AL易位系（T6VS/6AL）组成，其中大部分携带Yr26和Pm21基因，起源于贵州。此外，基于模型的群体结构分析显示了广泛的混合，并根据来源、系谱或抗病性将这些小麦进一步划分为6个亚群（SG1、SG2、SG3、SG4、SG5和SG6）。这些信息对中国西南地区小麦育种和今后利用这些小麦种质资源进行抗病关联作图具有重要意义。
Understanding genetic variability in existing wheat accessions is critical for collection, conservation and use of wheat germplasms. In this study, 138 Chinese southwest wheat accessions were investigated by genotyping using two resistance gene makers (Pm21 and Yr26) and DArT-seq technique. Finally, about 50% cultivars (lines) amplified the specific allele for the Yr26 gene (Gwm11) and 40.6% for the Pm21 gene (SCAR1265). By DArT-seq analysis, 30,485 markers (6486 SNPs and 23999 DArTs) were obtained with mean polymorphic information content (PIC) value 0.33 and 0.28 for DArT and SNP marker, respectively. The mean Dice genetic similarity coefficient (GS) was 0.72. Two consistent groups of wheat varieties were identified using principal coordinate analysis (PCoA) at the level of both the chromosome 6AS and the whole-genome, respectively. Group I was composed of non-6VS/6AL translocation lines of different origins, while Group II was composed of 6VS/6AL translocation (T6VS/6AL) lines, most of which carried the Yr26 and Pm21 genes and originated from Guizhou. Besides, a model-based population structure analysis revealed extensive admixture and further divided these wheat accessions into six subgroups (SG1, SG2, SG3, SG4, SG5 and SG6), based on their origin, pedigree or disease resistance. This information is useful for wheat breeding in southwestern China and association mapping for disease resistance using these wheat germplasms in future.